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1.
Zhonghua Yi Xue Za Zhi ; 98(44): 3607-3612, 2018 Nov 27.
Artículo en Zh | MEDLINE | ID: mdl-30486579

RESUMEN

Objective: To evaluate the performance of MALDI Biotyper system in identification of clinically isolated pathogens so as to provide a new rapid identification method. Methods: Total 21 270 pathogens strains, isolated from the First Affiliated Hospital of Fujian Medical Universityduring Nov. 2015 to Dec. 2016, were identified by VITEK-Ⅱ, API and MALDI Biotyper system, respectively.The isolated strains were confirmed by DNA sequencing. Results: The identification of common bacteria with MALDI Biotyper and phenotypic system is highly consistent (>95% and >90%). Among 43 strains of anaerobic bacteria, MALDI Biotyper could identify 90.7% bacteria to species level and 97.7% bacteria to genus level with the statistical significance(χ(2)=6.76, P<0.01), while phenotypic system only identified 65.1% bacteria to species and 69.8% bacteria to genus. Also, no statistical significance was shown for Trichosporon and Candida(P>0.05). MALDI Biotyper could identify 76% filamentous fungi and all of Actinomycetes, Nocardia, Mycobacterium and Legionella to genus level. Conclusions: MALDI Biotyper is an easy-performed, sensitive method for the identification of clinically isolated pathogens. Additionally, the pretreatment and reference database has the effect on identification.


Asunto(s)
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Hongos , Legionella , Mycobacterium , Análisis de Secuencia de ADN
2.
J Viral Hepat ; 22(4): 441-52, 2015 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-25278170

RESUMEN

We aimed to investigate the ability of HBsAg plasma level kinetics to predict therapy response by studying 23 children with infancy-acquired chronic hepatitis B (CHB) during combination sequential therapy with lead-in lamivudine (LAM) and add-on interferon-α (IFN-α) [5 responders (R = anti-HBs seroconversion) and 18 nonresponders (NR)] and to assess their relationship with pretreatment intrahepatic HBV-DNA and cccDNA and HBsAg and HBcAg liver expression. Plasma HBsAg levels were measured in samples before (treatment week 0 = TW0), during (TW9, TW28, TW52) and after (follow-up week = FUW24) therapy by Abbott ARCHITECT(®) assay [log10 IU/mL]. Baseline liver HBV-DNA and cccDNA were quantified by real-time TaqMan PCR [log10 copies/ng genomic DNA]. HBsAg and HBcAg liver expression was evaluated by immunostaining of formalin-fixed, paraffin-embedded specimens [number of positive cells/1000 hepatocytes]. All results are presented as medians. Plasma: at baseline, on-treatment and during follow-up, HBsAg levels were lower in R than NR (TW0: 4.36 vs 4.75;TW28: 2.44 vs 4.35;TW52: 0 vs 4.08 and FUW24: 0.17 vs 4.35, all P < 0.05). Liver: baseline HBV-DNA (3.82 vs 4.71, P = 0.16) and cccDNA (1.98 vs 2.26, P = 0.18) tended to be lower in R than NR, HBsAg expression was lower in R than NR (0.5 vs 4.7, P = 0.03), and HBcAg expression was similar between R and NR. There were positive correlations between plasma HBsAg levels and liver HBV-DNA (r = 0.44, P = 0.04), cccDNA (r = 0.41, P = 0.04) and HBsAg liver expression (r = 0.38, P = 0.05). Lower baseline HBsAg plasma levels, lower HBsAg expression in liver and on-treatment decline of plasma HBsAg levels heralds HBsAg clearance and response to treatment in tolerant children with CHB.


Asunto(s)
Antivirales/uso terapéutico , Biomarcadores/sangre , Monitoreo de Drogas/métodos , Antígenos de Superficie de la Hepatitis B/sangre , Hepatitis B Crónica/tratamiento farmacológico , Adolescente , Niño , Preescolar , ADN Viral/análisis , Quimioterapia Combinada/métodos , Femenino , Perfilación de la Expresión Génica , Antígenos de Superficie de la Hepatitis B/análisis , Humanos , Inmunohistoquímica , Interferón-alfa/uso terapéutico , Lamivudine/uso terapéutico , Hígado/virología , Masculino , Plasma/química , Pronóstico , Resultado del Tratamiento
3.
J Pediatr Gastroenterol Nutr ; 60(2): 159-64, 2015 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-25304891

RESUMEN

OBJECTIVE: Autoimmune hepatitis (AIH) is considered an underdiagnosed cause of fulminant hepatic failure (FHF). Autoimmune FHF (AI-FHF) is believed to lead invariably to liver transplantation (LTX) or death. We aimed to describe the autoimmune features of children diagnosed as having AI-FHF and indeterminate FHF (ID-FHF), and describe the outcome of patients with AI-FHF treated with immunosuppressive drugs. METHODS: In this case-control study, the files of patients with AI-FHF and ID-FHF were reviewed and compared. AIH was diagnosed based on positive autoantibodies, raised immunoglobulin G, and histology when available. FHF was defined by raised transaminases, international normalised ratio ≥ 2.0, presence of encephalopathy, and no previously recognised liver disease. RESULTS: A total of 46 children with FHF were managed in the last 15 years: 10/46 (22%) had AI-FHF, 20/46 (43%) ID-FHF, and 16 had other diagnosis. The mean follow-up time was 4.6 years. AI-FHF and ID-FHF differed for the presence of autoantibodies (10/10, 6/10 liver/kidney microsome [LKM]-type, vs 3/20, none LKM, P < 0.0001), immunoglobulin G level (1845 vs 880 mg/dL, P < 0.001), median age at diagnosis (6.4 vs 1.8 years, P = 0.017), and alanine aminotransferase level (1020 vs 2386 IU/L, P = 0.029). Liver histology did not allow to differentiate the 2 conditions. Among the patients with AI-FHF, 4/9 who received steroids recovered; 5/9 required LTX and 1 died awaiting treatment. CONCLUSIONS: AIH is a much more common cause of FHF than previously suggested, and a complete autoantibody testing including LKM-type is essential in this setting. Autoantibodies are uncommon in ID-FHF, and histology cannot distinguish it from AI-FHF. A cautious steroid trial may avoid LTX in some of the patients with AI-FHF.


Asunto(s)
Hepatitis Autoinmune/complicaciones , Inmunosupresores/uso terapéutico , Fallo Hepático Agudo/etiología , Fallo Hepático Agudo/terapia , Adolescente , Factores de Edad , Alanina Transaminasa/sangre , Anticuerpos Antinucleares/sangre , Autoanticuerpos/sangre , Azatioprina/uso terapéutico , Estudios de Casos y Controles , Niño , Preescolar , Ciclosporina/uso terapéutico , Estudios de Seguimiento , Humanos , Inmunoglobulina G/sangre , Lactante , Fallo Hepático Agudo/patología , Trasplante de Hígado , Metilprednisolona/uso terapéutico , Estudios Retrospectivos , Tasa de Supervivencia
4.
Surg Oncol ; 49: 101965, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37348195

RESUMEN

AIMS: Minor hepatectomy, which is increasingly carried out laparoscopically (LLR), is a cornerstone of curative treatment for hepatocellular carcinoma (HCC). The majority of relevant publications however originate from regions with endemic viral hepatitis. Although the incidence of HCC in the UK is increasing, little is known about outcomes following LLR. METHODS: Consecutive patients undergoing minor (involving ≤2 segments) LLR or open resection (OLR) at our institute between 2014 and 2021 were compared. Selection from a plethora of factors potentially impacting on overall (OS) and disease free survival (DFS) was optimised with Lasso regression. To enable analysis of patients having repeat resection, multivariate frailty modelling was utilised to calculate hazard ratios (HR). RESULTS: The analysis of 111 liver resections included 55 LLR and 56 OLR. LLR was associated with a shorter hospital stay (5 ± 2 vs. 7 ± 2 days; p < 0.001) and a lower comprehensive complication index (4.43 vs. 9.96; p = 0.006). Mean OS (52.3 ± 2.3 vs. 49.9 ± 3.0 months) and DFS (33.9 ± 3.4 vs. 36.5 ± 3.6 months; p = 0.59) were comparable between LLR and OLR, respectively (median not reached). Presence of mixed cholangiocarcinoma/HCC, satellite lesions and AFP level predicted OS and DFS. In addition tumour size was predictive of DFS. CONCLUSIONS: In the studied population minor LLR was associated with shorter hospital stay and fewer complications while offering non-inferior long-term outcomes. A number of predictors for disease free survival have been elucidated that may aid in identifying patients with a high risk of disease recurrence and need for further treatment.


Asunto(s)
Carcinoma Hepatocelular , Laparoscopía , Neoplasias Hepáticas , Humanos , Carcinoma Hepatocelular/patología , Hepatectomía , Neoplasias Hepáticas/patología , Estudios Retrospectivos , Laparoscopía/efectos adversos , Complicaciones Posoperatorias/etiología , Reino Unido/epidemiología , Tiempo de Internación
5.
Br J Dermatol ; 162(5): 1098-102, 2010 May.
Artículo en Inglés | MEDLINE | ID: mdl-19995364

RESUMEN

BACKGROUND: Extramammary Paget disease is an uncommon skin tumour occurring mostly in the genitoperineal region. Previous reports have shown frequent expression of androgen receptor, suggesting a tumour-proliferative effect of androgens on Paget cells. Androgen-converting enzymes such as 5alpha-reductase, which locally produces a bioactive androgen, have recently gained attention in studies of the intratumoral actions of androgens. OBJECTIVES: We investigated correlations between the androgenic microenvironment and invasiveness in extramammary Paget disease, particularly in terms of sex differences. METHODS: We examined 58 cases of extramammary Paget disease (32 men, 26 women; 42 noninvasive, 16 invasive) using immunohistochemistry for androgen receptor and 5alpha-reductase. RESULTS: In all 58 cases, expression rates were 57% for androgen receptor and 55% for 5alpha-reductase, with 38% double-positivity for androgen receptor and 5alpha-reductase. Only 5alpha-reductase expression rate was significantly higher in invasive cases (81%) than in noninvasive cases (45%; P = 0.042). For invasive cases, numbers of double-positive results for androgen receptor and 5alpha-reductase were significantly higher in men (70%) than in women (17%; P = 0.039). CONCLUSIONS: Double positivity for androgen receptor and 5alpha-reductase in Paget cells suggests autocrine synthesis of androgens in extramammary Paget disease. The different hormonal microenvironments in male and female cases and intratumoral androgen levels affect the invasiveness of extramammary Paget disease.


Asunto(s)
Colestenona 5 alfa-Reductasa/metabolismo , Enfermedad de Paget Extramamaria/metabolismo , Receptores Androgénicos/metabolismo , Neoplasias Cutáneas/metabolismo , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad , Invasividad Neoplásica , Proteínas de Neoplasias/metabolismo , Enfermedad de Paget Extramamaria/patología , Enfermedad de Paget Extramamaria/secundario , Factores Sexuales , Neoplasias Cutáneas/patología
6.
Ann Rheum Dis ; 68(8): 1310-5, 2009 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-18701557

RESUMEN

BACKGROUND: Mikulicz's disease (MD) has been considered as one manifestation of Sjögren's syndrome (SS). Recently, it has also been considered as an IgG(4)-related disorder. OBJECTIVE: To determine the differences between IgG(4)-related disorders including MD and SS. METHODS: A study was undertaken to investigate patients with MD and IgG(4)-related disorders registered in Japan and to set up provisional criteria for the new clinical entity IgG(4)-positive multiorgan lymphoproliferative syndrome (IgG(4)+MOLPS). The preliminary diagnostic criteria include raised serum levels of IgG(4) (>135 mg/dl) and infiltration of IgG(4)(+) plasma cells in the tissue (IgG(4)+/IgG+ plasma cells >50%) with fibrosis or sclerosis. The clinical features, laboratory data and pathologies of 64 patients with IgG(4)+MOLPS and 31 patients with typical SS were compared. RESULTS: The incidence of xerostomia, xerophthalmia and arthralgia, rheumatoid factor and antinuclear, antiSS-A/Ro and antiSS-B/La antibodies was significantly lower in patients with IgG(4)+MOLPS than in those with typical SS. Allergic rhinitis and autoimmune pancreatitis were significantly more frequent and total IgG, IgG(2), IgG(4) and IgE levels were significantly increased in IgG(4)+MOLPS. Histological specimens from patients with IgG(4)+MOLPS revealed marked IgG(4)+ plasma cell infiltration. Many patients with IgG(4)+MOLPS had lymphocytic follicle formation, but lymphoepithelial lesions were rare. Few IgG(4)+ cells were seen in the tissue of patients with typical SS. Thirty-eight patients with IgG(4)+MOLPS treated with glucocorticoids showed marked clinical improvement. CONCLUSION: Despite similarities in the involved organs, there are considerable clinical and pathological differences between IgG(4)+MOLPS and SS. Based on the clinical features and good response to glucocorticoids, we propose a new clinical entity: IgG(4)+MOLPS.


Asunto(s)
Inmunoglobulina G/análisis , Trastornos Linfoproliferativos/inmunología , Enfermedad de Mikulicz/inmunología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Biopsia , Diagnóstico Diferencial , Femenino , Glucocorticoides/uso terapéutico , Humanos , Aparato Lagrimal/patología , Trastornos Linfoproliferativos/diagnóstico , Trastornos Linfoproliferativos/tratamiento farmacológico , Trastornos Linfoproliferativos/patología , Imagen por Resonancia Magnética , Masculino , Persona de Mediana Edad , Enfermedad de Mikulicz/diagnóstico , Enfermedad de Mikulicz/tratamiento farmacológico , Enfermedad de Mikulicz/patología , Prednisolona/uso terapéutico , Estudios Retrospectivos , Glándulas Salivales Menores/patología , Síndrome de Sjögren/diagnóstico , Síndrome de Sjögren/inmunología , Síndrome de Sjögren/patología , Síndrome , Adulto Joven
8.
Kyobu Geka ; 62(1): 82-5, 2009 Jan.
Artículo en Japonés | MEDLINE | ID: mdl-19195192

RESUMEN

A 24-year-old male admitted to our hospital with a pulmonary nodule detected by his chest X-ray and computed tomography (CT). His laboratory findings were within normal limits. Chest CT showed a 10 mm solitary nodule in the right lower lobe. Positron emission tomography showed moderately positive detection correspond to the nodule. We couldn't rule out a malignant tumor and performed partial resection of the right lower lobe. Pathological findings definitely revealed pulmonary inflammatory myofibroblastic tumor. This case was reported together with some reviews of the literature.


Asunto(s)
Neoplasias Pulmonares/patología , Neoplasias de Tejido Muscular/patología , Humanos , Masculino , Adulto Joven
9.
Clin Exp Immunol ; 152(3): 432-9, 2008 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-18422733

RESUMEN

Mikulicz's disease (MD) is gaining acceptance as an immunoglobulin G4 (IgG4)-related disease characterized by bilateral lacrimal and salivary gland swelling. The aetiology of MD and other IgG4-related diseases is still unclear. The present work was performed to study the clonality of infiltrating IgG4-positive plasma cells in lacrimal glands and circulating peripheral blood cells in patients with MD, and compare the clonal relationship between infiltrating and circulating IgG4 positive cells. Total cellular RNA was extracted from the lacrimal glands and peripheral blood in five MD patients. Reverse transcription polymerase chain reaction was performed with primers specific for activation-induced cytidine deaminase (AID) and for Ig VH and IgG4. Sequences of Ig VH were compared with the structure of Ig VH of the lacrimal glands and the peripheral blood cells. AID was expressed to varying degrees in lacrimal glands of all MD patients. Most IgG4-positive cells infiltrating lacrimal glands and in peripheral blood were polyclonal, although several clonally related pairs were detected. In one patient, two of the circulating IgG4 VH4-59 clones shared identical CDR3 sequences with the clones within the lacrimal glands. In conclusion, while most tissue-infiltrating and circulating IgG4-positive cells in MD are polyclonal, some clonally related IgG4 positive cells exist between lacrimal gland and peripheral blood, accounting for the clinical features of MD as an IgG4-related disease involving multiple organs.


Asunto(s)
Inmunoglobulina G/análisis , Aparato Lagrimal/inmunología , Subgrupos Linfocitarios/inmunología , Enfermedad de Mikulicz/inmunología , Células Plasmáticas/inmunología , Anciano , Secuencia de Aminoácidos , Células Clonales/inmunología , Regiones Determinantes de Complementariedad/genética , Citidina Desaminasa/metabolismo , Femenino , Genes de las Cadenas Pesadas de las Inmunoglobulinas , Humanos , Aparato Lagrimal/enzimología , Masculino , Persona de Mediana Edad , Enfermedad de Mikulicz/enzimología , Enfermedad de Mikulicz/genética , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Hipermutación Somática de Inmunoglobulina
10.
Kyobu Geka ; 61(9): 779-83, 2008 Aug.
Artículo en Japonés | MEDLINE | ID: mdl-18697460

RESUMEN

A 68-year-old male suffered from right pneumothorax and was admitted to our hospital. He had a previous history of angiosarcoma of the scalp, and had received local resection and chemoradiotherapy. Chest computed tomography (CT) on admission revealed right pneumothorax and bilateral multiple thin-walled cavities of the lung. We performed partial resection of right lung. Histopathological examination showed a small metastatic lesion around the thin-walled cavities of the lung. Four months after the 1st lung resection, he suffered left pneumothorax. We performed partial resection of the left lung. Ten days after the 2nd lung resection, left pneumothorax recurred. Nine days later, he also developed right pneumothorax. We performed the 3rd operation for right lung. Thoracoscopy demonstrated multiple bullas in right lung and it showed impossibility for radical surgery. Although surgical resection for pneumothorax secondary to metastatic lung cancer is usually efficient, it is very hard to manage the pneumothorax of metastatic angiosarcoma.


Asunto(s)
Hemangiosarcoma/patología , Neoplasias Pulmonares/complicaciones , Neoplasias Pulmonares/secundario , Neumotórax/etiología , Cuero Cabelludo , Neoplasias Cutáneas/patología , Anciano , Humanos , Masculino
12.
Ann R Coll Surg Engl ; 96(5): e18-20, 2014 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-24992406

RESUMEN

INTRODUCTION: Incidental gallbladder cancer is found in 0.6-2.1% of patients undergoing laparoscopic cholecystectomy for symptomatic gallstones. Patients with Tis or T1a tumours generally undergo no further intervention. However, spilled stones during surgery may have catastrophic consequences. We present a case and suggest aggressive management in patients with incidental gallbladder cancer who had spilled gallstones at surgery. CASE HISTORY: A 37-year-old woman underwent a laparoscopic cholecystectomy for symptomatic gallstones, during which some stones were spilled into the peritoneal cavity. Subsequent histological examination confirmed incidental pT1a gallbladder cancer. Hepatopancreatobiliary multidisciplinary team discussion agreed on regular six-monthly follow-up. The patient developed recurrent pain two years after surgery. Computed tomography revealed a lesion in segment 6 of the liver. At laparotomy, multiple tumour embedded gallstones were found on the diaphragm. Histological examination showed features (akin to the original pathology) consistent with a metastatic gallbladder tumour. CONCLUSIONS: This case highlights the potential for recurrence of early stage disease resulting from implantation of dysplastic or malignant cells carried through spilled gallstones. It is therefore important to know if stones were spilled during original surgery in patients with incidental gallbladder cancer following a laparoscopic cholecystectomy. Aggressive and early surgical management should be considered for these patients.


Asunto(s)
Adenocarcinoma/diagnóstico , Colecistectomía Laparoscópica/métodos , Neoplasias de la Vesícula Biliar/diagnóstico , Cálculos Biliares/cirugía , Adenocarcinoma/complicaciones , Adenocarcinoma/secundario , Adulto , Enfermedades de las Vías Biliares/etiología , Cólico/etiología , Resultado Fatal , Femenino , Neoplasias de la Vesícula Biliar/complicaciones , Cálculos Biliares/complicaciones , Humanos , Hallazgos Incidentales , Complicaciones Intraoperatorias/etiología , Neoplasias Hepáticas/secundario , Recurrencia Local de Neoplasia/etiología , Siembra Neoplásica
13.
Acta Biomater ; 7(4): 1928-33, 2011 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-21115142

RESUMEN

Deep infection remains a serious complication in orthopedic implant surgery. In order to reduce the incidence of implant-associated infections, several biomaterial surface treatments have been proposed. This study focused on evaluating the antibacterial activity of iodine-supported titanium (Ti-I(2)) and its impact on post-implant infection, as well as determining the potential suitability of Ti-I(2) as a biomaterial. External fixation pins were used in this experiment as trial implants because of the ease of making the septic models. The antibacterial activity of the metal was measured using a modification of the Japanese Industrial Standards method. Activity was evaluated by exposing the implants to Staphylococcus aureus or Escherichia coli and comparing reaction of pathogens to Ti-I(2) vs. stainless steel and titanium controls. Ti-I(2) clearly inhibited bacterial colonization more than the control metals. In addition, cytocompatibility was assessed by counting the number of colonies that formed on the metals. The three metals showed the same amount of fibroblast colony formation. Japanese white rabbits were used as an in vivo model. Three pins were inserted into both femora of six rabbits for histological analysis. Pin sites were inspected and graded for infection and inflammation. Fewer signs of infection and inflammatory changes were observed in conjunction with the Ti-I(2) pins. Furthermore, osteoconductivity of the implant was evaluated with osteoid formation surface of the pin. Consecutive bone formation was observed around the Ti-I(2) and titanium pins, while little osteoid formation was found around the stainless steel pins. These findings suggest that Ti-I(2) has antimicrobial activity and exhibits cytocompatibility. Therefore, Ti-I(2) substantially reduces the incidence of implant infection and shows particular promise as a biomaterial.


Asunto(s)
Antibacterianos/farmacología , Yodo/farmacología , Prótesis e Implantes , Titanio/farmacología , Animales , Recuento de Colonia Microbiana , Escherichia coli/efectos de los fármacos , Inflamación/patología , Pruebas de Sensibilidad Microbiana , Osteogénesis/efectos de los fármacos , Conejos , Staphylococcus aureus/efectos de los fármacos , Staphylococcus aureus/crecimiento & desarrollo
15.
Oncogene ; 28(14): 1725-35, 2009 Apr 09.
Artículo en Inglés | MEDLINE | ID: mdl-19234486

RESUMEN

Tumor metastasis is a complex phenomenon that is the culmination of effects of numerous cellular factors. We have shown that the Epstein-Barr virus (EBV) oncoprotein, latent membrane protein 1 (LMP1), is capable of inducing a wide range of such factors in cell culture, expression of which is also elevated in the LMP1-expressing tumor, nasopharyngeal carcinoma (NPC), a highly invasive neoplasm. Recently, the membrane crosslinker protein, ezrin, has been implicated in tumor cell metastasis and malignant progression. In this study, we evaluated the possible role of LMP1 and ezrin in the pathophysiology of NPC. We show that C-terminal phosphorylation of ezrin is increased by the expression of LMP1 in nasopharyngeal (NP) cells through a protein kinase C (PKC) pathway. LMP1 enhances the organization of a ternary complex of CD44, ezrin and F-actin, which is a prerequisite for ezrin phosphorylation. In NPC tissues, the expression of phosphoezrin and LMP1 is directly correlated. Silencing of endogenously expressed ezrin suppresses LMP1-induced cell motility and invasiveness. Moreover, the inhibition of ezrin phosphorylation by PKC inhibitor suppresses migration and invasion of NP cells. These data show that the phosphorylation of ezrin and its recruitment to the cell membrane linked to F-actin and CD44 is a process required for LMP1-stimulated cell motility and invasion of NP cells.


Asunto(s)
Proteínas del Citoesqueleto/fisiología , Neoplasias Nasofaríngeas/patología , Proteínas de la Matriz Viral/fisiología , Línea Celular Tumoral , Membrana Celular/metabolismo , Movimiento Celular , Citosol/metabolismo , Proteínas de Unión al ADN/metabolismo , Humanos , Receptores de Hialuranos/análisis , Invasividad Neoplásica , Fosforilación , Proteína Quinasa C/fisiología , Transporte de Proteínas , Factores de Transcripción/metabolismo , Quinasas Asociadas a rho/fisiología
16.
Oncogene ; 28(32): 2910-8, 2009 Aug 13.
Artículo en Inglés | MEDLINE | ID: mdl-19503097

RESUMEN

The partition-defective 3 (PAR-3) protein is implicated in the formation of tight junctions at epithelial cell-cell contacts. We investigated DNA copy number aberrations in human esophageal squamous cell carcinoma (ESCC) cell lines using a high-density oligonucleotide microarray and found a homozygous deletion of PARD3 (the gene encoding PAR-3). Exogenous expression of PARD3 in ESCC cells lacking this gene enhanced the recruitment of zonula occludens 1 (ZO-1), a marker of tight junctions, to sites of cell-cell contact. Conversely, knockdown of PARD3 in ESCC cells expressing this gene caused a disruption of ZO-1 localization at cell-cell borders. A copy number loss of PARD3 was observed in 15% of primary ESCC cells. Expression of PARD3 was significantly reduced in primary ESCC tumors compared with their nontumorous counterparts, and this reduced expression was associated with positive lymph node metastasis and poor differentiation. Our results suggest that deletion and reduced expression of PARD3 may be a novel mechanism that drives the progression of ESCC.


Asunto(s)
Carcinoma de Células Escamosas/genética , Proteínas de Ciclo Celular/genética , Neoplasias Esofágicas/genética , Perfilación de la Expresión Génica , Regulación Neoplásica de la Expresión Génica , Proteínas de la Membrana/genética , Proteínas Adaptadoras Transductoras de Señales , Anciano , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patología , Proteínas de Ciclo Celular/metabolismo , Línea Celular Tumoral , Movimiento Celular , Neoplasias Esofágicas/metabolismo , Neoplasias Esofágicas/patología , Eliminación de Gen , Dosificación de Gen , Homocigoto , Humanos , Immunoblotting , Lactante , Uniones Intercelulares/metabolismo , Masculino , Proteínas de la Membrana/metabolismo , Microscopía Confocal , Microscopía Fluorescente , Persona de Mediana Edad , Análisis de Secuencia por Matrices de Oligonucleótidos , Fosfoproteínas/metabolismo , Interferencia de ARN , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Proteína de la Zonula Occludens-1
17.
Histopathology ; 51(3): 390-400, 2007 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-17553067

RESUMEN

AIMS: The histogenesis and biological behaviour of peripheral intrahepatic cholangiocarcinoma (peripheral CC) remain unclarified. The aim of this study was to examine the growth pattern of peripheral CC (24 cases) in comparison with hepatocellular carcinoma (HCC, 27 cases) and metastatic colorectal adenocarcinoma (MCA, 24 cases). METHODS AND RESULTS: Tumour/surrounding liver borders were classified as: (i) fibrous encapsulation, (ii) compressive growth, and (iii) infiltrating replacement. Nineteen of 24 peripheral CCs showed (iii), whereas 23 of 27 HCCs showed (i) and 17 of 24 MCAs showed (ii). In (iii), carcinoma cells infiltrated the surrounding liver without compression, and hepatic supporting vascular structures such as portal tracts were secondarily incorporated into the tumour. In (i) and (ii), the surrounding liver was compressed and no or few portal tracts were incorporated within the tumour. Fifteen of 24 peripheral CCs were composed of carcinoma cells resembling reactive bile ductules and these cells were positive for neural cell adhesion molecule (NCAM), a marker of proliferating bile ductules. The remaining nine peripheral CCs were composed of ordinary adenocarcinoma and negative for NCAM. CONCLUSIONS: A subgroup of peripheral CCs with an infiltrating replacement growth pattern resembles reactive bile ductules and expresses NCAM. 'Bile ductular carcinoma' may be a better term for this subgroup.


Asunto(s)
Neoplasias de los Conductos Biliares/patología , Conductos Biliares Intrahepáticos/patología , Conductos Biliares/patología , Colangiocarcinoma/patología , Adenocarcinoma/metabolismo , Adenocarcinoma/secundario , Anciano , Anciano de 80 o más Años , Autopsia , Neoplasias de los Conductos Biliares/metabolismo , Conductos Biliares/metabolismo , Conductos Biliares Intrahepáticos/química , Antígeno CD56/análisis , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patología , Colangiocarcinoma/metabolismo , Neoplasias Colorrectales/metabolismo , Neoplasias Colorrectales/secundario , Femenino , Humanos , Inmunohistoquímica , Queratinas/análisis , Hígado/química , Hígado/patología , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patología , Masculino , Persona de Mediana Edad , Moléculas de Adhesión de Célula Nerviosa/análisis , Tamaño de los Órganos , Vimentina/análisis
18.
Histopathology ; 49(5): 466-78, 2006 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-17064292

RESUMEN

AIMS: To define a new histological staging and grading system for primary biliary cirrhosis (PBC), to provide more information reflecting clinical laboratory data and the prognosis to hepatologists. METHODS AND RESULTS: First, 17 histological lesions of PBC were scored in 188 needle liver biopsy specimens. Factor analysis yielded three independent groups of factors: factor 1 (fibrosis, fibrous piecemeal necrosis, orcein-positive granules, bile plugs, Mallory bodies, feathery degeneration, bile duct loss and atypical ductular proliferation); factor 2 (portal inflammation, eosinophilic infiltration, lymphoid follicles, epithelioid granulomas, interface hepatitis and chronic cholangitis); and factor 3 (interface hepatitis, lobular hepatitis, acidophilic bodies and pigmented macrophages). The eight findings of factor 1, but not factors 2 and 3, were significantly correlated with clinical laboratory data and scores in the Mayo Clinic's prognostic model. Factor 1 lesions may reflect histological progression (staging), while factor 2 and 3 lesions may relate to necroinflammatory activity (grading). Then, we devised a staging and grading system using three lesions (bile duct loss, fibrosis and orcein-positive granules) from factor 1 and three from factors 2 and 3 (chronic cholangitis, interface hepatitis and lobular hepatitis). CONCLUSION: This new system might provide more pathological information on PBC patients for hepatologists.


Asunto(s)
Cirrosis Hepática Biliar/clasificación , Cirrosis Hepática Biliar/patología , Hígado/patología , Biopsia con Aguja , Progresión de la Enfermedad , Humanos , Cirrosis Hepática Biliar/fisiopatología , Pronóstico
19.
Hua Xi Yi Ke Da Xue Xue Bao ; 21(3): 293-7, 1990 Sep.
Artículo en Zh | MEDLINE | ID: mdl-2093069

RESUMEN

Four systems were studied used for chemiluminescent determination of HRP and the determination conditions optimized. In luminol-H2O2 (NaOH) system, HRP was determined conveniently in the range of 0.05-4 pmol, with a correlation coefficient of 0.998 for the linear log-log equation and a limit of detection of 16 fmol HRP. In pyrogallol-H2O2 (pH 6.5) system, 0.1-100 pmol of HRP were determined, based on three log-log regression equations with correlation coefficients of 0.997, 0.994 and 0.999, respectively, and limits of detection of 3-17 fmol HRP. Luminol-H2O2-para iodophenol system was used to determine HRP in the range to 10-50 fmol, based on the chemiluminescence intensity at 1 min and the correlation coefficient of the log-log equation being 0.996 with a limit of detection of 10 fmol HRP. When p-hydroxybiphenyl was used to take the place of p-iodophenol and the chemiluminescence intensity at 1 min or 3 min measured, 6-40 fmol HRP were determined with a correlation coefficient of 0.994 or 0.995 for the log-log equation and a limit of detection of 6.5 or 3.3 fmol HRP.


Asunto(s)
Peroxidasa de Rábano Silvestre/análisis , Mediciones Luminiscentes , Métodos
20.
Mod Pathol ; 14(12): 1304-9, 2001 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-11743055

RESUMEN

Biliary cystadenocarcinoma with oncocytic differentiation was first reported in 1992. This is a report of a second case. The patient (a 71-year-old man) was admitted to our hospital complaining of abdominal fullness. Multicystic lesions were identified in the left hepatic lobe radiologically. The patient died of peritoneal dissemination of carcinoma 20 months later. At autopsy, the tumor of the left hepatic lobe was found to be composed of adjoining multiple cystic lesions and a solid lesion with infiltration of the hepatic hilus and peritoneal dissemination. Histologically, the multicystic lesions were covered by papillary neoplastic epithelial cells with an eosinophilic granular cytoplasm resembling that of oncocytes and a fine fibrovascular core. The cyst wall was fibrous, but there was no mesenchymal stroma. In the solid lesion and infiltrated areas, acidophilic and granular carcinoma cells formed small glandular or solid cord patterns with much mucin secretion (mucinous carcinoma). Immunohistochemically, carcinoma cells of both components were found to contain many mitochondria and showed the phenotypes of hepatocytes and cholangiocytes. Interestingly, the intrahepatic biliary tree also was invaded by carcinoma cells. This may be a case of intraductal oncocytic papillary neoplasm of the left hepatic lobe followed by secondary cystic dilatation of the affected bile duct.


Asunto(s)
Adenoma Oxifílico/secundario , Neoplasias de los Conductos Biliares/patología , Conductos Biliares Intrahepáticos/patología , Cistadenocarcinoma Papilar/secundario , Neoplasias Hepáticas/patología , Células Oxífilas/patología , Adenoma Oxifílico/química , Adenoma Oxifílico/diagnóstico por imagen , Anciano , Neoplasias de los Conductos Biliares/química , Neoplasias de los Conductos Biliares/diagnóstico por imagen , Biomarcadores de Tumor/análisis , Cistadenocarcinoma Papilar/química , Cistadenocarcinoma Papilar/diagnóstico por imagen , Resultado Fatal , Humanos , Neoplasias Hepáticas/química , Neoplasias Hepáticas/diagnóstico por imagen , Masculino , Proteínas de Neoplasias/análisis , Tomografía Computarizada por Rayos X
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