Detection of captan residues in apple juice using fluorescence spectroscopy combined with a genetic algorithm and support vector machines.

The captan residues in apple juice were detected by fluorescence spectrometry, and the content level of captan was predicted based on a genetic algorithm and support vector machines (GA-SVMs). According to the captan concentration in apple juice, the experimental samples were divided into four levels, including no excess, slight excess, moderate excess, and severe excess. A GA was used to select the characteristic wavelength and optimize SVM parameters, and SVM was applied to train the classification model. 50 characteristic wavelength points were selected from the original fluorescence spectra, which contained 401 wavelength points, and the classification accuracy of the training set and test set is 99.02% and 100%, respectively, which is higher than the traditional PLS method. The results show that a GA can effectively select the feature wavelengths, and an SVM model can accurately predict the content level of captan residues. A fast and non-destructive analysis method, combined with a GA and SVM based on fluorescence spectroscopy, was realized.

Highly Efficient Spatial-Temporal Correlation Basis for 5G IoT Networks.

One of the major concerns in 5G IoT networks is that most of the sensor nodes are powered through limited lifetime, which seriously affects the performance of the networks. In this article, Compressive sensing (CS) technique is used to decrease transmission cost in 5G IoT networks. Sparse basis is one of the important steps in the CS. However, most of the existing sparse basis-based method such as DCT (Discrete cosine transform) and DFT (Discrete Fourier Transform) basis do not capture data structure characteristics in the networks. They also do not take into consideration multi-resolution representations. In addition, some of sparse basis-driven methods exploit either spatial or temporal features, resulting in performance degradation of CS-based strategies. To address these challenging problems, we propose a novel spatial-temporal correlation basis algorithm (SCBA). Subsequently, an optimal basis algorithm (OBA) is provided considering greedy scoring criteria. To evaluate the efficiency of OBA, orthogonal wavelet basis algorithm (OWBA) by employing NS (Numerical Sparsity) and GI (Gini Index) sparse metrics is also presented. In addition, we discuss the complexity of the above three algorithms, and prove that OBA has low numerical rank. After experimental evaluation, we found that OBA is capable of the sparsest representing original signal compared to spatial, DCT, haar-1, haar-2, and rbio5.5. Furthermore, OBA has the low recovery error and the highest efficiency.

Gp85 protein encapsulated by alginate-chitosan composite microspheres induced strong immunogenicity against avian leukosis virus in chicken.

Introduction: Avian leukosis, a viral disease affecting birds such as chickens, presents significant challenges in poultry farming due to tumor formation, decreased egg production, and increased mortality. Despite the absence of a commercial vaccine, avian leukosis virus (ALV) infections have been extensively documented, resulting in substantial economic losses in the poultry industry. This study aimed to develop alginate-chitosan composite microspheres loaded with ALV-J Gp85 protein (referred to as aCHP-gp85) as a potential vaccine candidate. Methods: Sodium alginate and chitosan were utilized as encapsulating materials, with the ALV-J Gp85 protein serving as the active ingredient. The study involved 45 specific pathogen-free (SPF) chickens to evaluate the immunological effectiveness of aCHP-gp85 compared to a traditional Freund adjuvant-gp85 vaccine (Freund-gp85). Two rounds of vaccination were administered, and antibody levels, mRNA expression of immune markers, splenic lymphocyte proliferation, and immune response were assessed. An animal challenge experiment was conducted to evaluate the vaccine's efficacy in reducing ALV-J virus presence and improving clinical conditions. Results: The results demonstrated that aCHP-gp85 induced a significant and sustained increase in antibody levels compared to Freund-gp85, with the elevated response lasting for 84 days. Furthermore, aCHP-gp85 significantly upregulated mRNA expression levels of key immune markers, notably TNF-α and IFN-γ. The application of ALV-J Gp85 protein within the aCHP-gp85 group led to a significant increase in splenic lymphocyte proliferation and immune response. In the animal challenge experiment, aCHP-gp85 effectively reduced ALV-J virus presence and improved clinical conditions compared to other groups, with no significant pathological changes observed. Discussion: The findings suggest that aCHP-gp85 elicits a strong and prolonged immune response compared to Freund-gp85, indicating its potential as an innovative ALV-J vaccine candidate. These results provide valuable insights for addressing avian leukosis in the poultry industry, both academically and practically.

Adaptive Image Enhancement Using Entropy-Based Subhistogram Equalization.

A novel image enhancement approach called entropy-based adaptive subhistogram equalization (EASHE) is put forward in this paper. The proposed algorithm divides the histogram of input image into four segments based on the entropy value of the histogram, and the dynamic range of each subhistogram is adjusted. A novel algorithm to adjust the probability density function of the gray level is proposed, which can adaptively control the degree of image enhancement. Furthermore, the final contrast-enhanced image is obtained by equalizing each subhistogram independently. The proposed algorithm is compared with some state-of-the-art HE-based algorithms. The quantitative results for a public image database named CVG-UGR-Database are statistically analyzed. The quantitative and visual assessments show that the proposed algorithm outperforms most of the existing contrast-enhancement algorithms. The proposed method can make the contrast of image more effectively enhanced as well as the mean brightness and details well preserved.

Image Enhancement via Subimage Histogram Equalization Based on Mean and Variance.

This paper puts forward a novel image enhancement method via Mean and Variance based Subimage Histogram Equalization (MVSIHE), which effectively increases the contrast of the input image with brightness and details well preserved compared with some other methods based on histogram equalization (HE). Firstly, the histogram of input image is divided into four segments based on the mean and variance of luminance component, and the histogram bins of each segment are modified and equalized, respectively. Secondly, the result is obtained via the concatenation of the processed subhistograms. Lastly, the normalization method is deployed on intensity levels, and the integration of the processed image with the input image is performed. 100 benchmark images from a public image database named CVG-UGR-Database are used for comparison with other state-of-the-art methods. The experiment results show that the algorithm can not only enhance image information effectively but also well preserve brightness and details of the original image.