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1.
Environ Sci Pollut Res Int ; 21(5): 3706-12, 2014 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-24281680

RESUMEN

This study evaluated the variability of GSTM1 and GSTT1 polymorphisms in individuals occupationally exposed to pesticides in ten Goias municipalities that present intense agricultural activity. We evaluated blood samples of 235 individuals, which 120 were rural workers occupationally exposed to pesticides and 115 formed the control group, analyzing GST polymorphisms by quantitative polymerase chain reaction (qPCR).The exposed group consisted of 111 men and nine women only getting an average of 39 ± 9 years. These workers were from ten rural municipalities situated at Goias state. It was found that 18 % of the exposed individuals had the GSTT1 null genotype and 49 % had the GSTM1 null genotype, and 10 % had both null genotypes. Data as intoxication (42 %), use of Personal Protection Equipment (PPE; 52 %) and if the worker prepared the pesticide (7 %), or if just applied the pesticide (22 %) or if the worker prepared and applied (71 %) have all been correlated with genetic polymorphisms. There were no statistically significant differences between the GSTM1 and GSTT1 polymorphisms between control and exposed groups. Finally, we could not associate a null GSTT1 or null GSTM1 polymorphisms or both to intoxication events caused by pesticides, but instead we presented the importance to use PPE to prevent such harm, once we found a statistically significant association between the use of PPE and events of intoxication (p ≤ 0.001).


Asunto(s)
Contaminantes Ocupacionales del Aire/toxicidad , Glutatión Transferasa/genética , Exposición Profesional/efectos adversos , Plaguicidas/toxicidad , Adulto , Agricultura , Consumo de Bebidas Alcohólicas/genética , Brasil , Femenino , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Polimorfismo Genético , Fumar/genética
2.
Environ Sci Pollut Res Int ; 20(10): 7334-40, 2013 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-23640389

RESUMEN

We evaluated 41 rural workers occupationally exposed to pesticides and 32 subjects as a control group, using the micronucleus (MN) and the comet assay. For the comet assay, we evaluated the peripheral blood, and for the MN, we sampled cells from the oral epithelium. Damage to DNA was measured by tail length, % DNA in tail (% tail), olive tail moment (OTM), and tail moment (TM). The exposed group presented an 8× increase in MN frequency, when compared to the control group (p <0.05). When we contrasted the MN frequencies between the individuals that use and do not use personal protective equipment, we found a mean of 7.5 MN (57 % variance) and 12.1 MN (130 % variance), respectively. The binucleated cells were 0.04 and 0.005, in the exposed and control groups, respectively, indicating 8× increase in the number of binucleated cells, when comparing the groups (p <0.05). In the comet assay, we demonstrated statistically significant differences in three parameters (% DNA, OTM, and TM) indicating that the rural workers presented high levels of genomic damages. Our results indicate that occupational exposure to pesticides could cause genome damage in somatic cells, representing a potential health risk to Brazilian rural workers that deal constantly with agrochemicals without adequate personal protection equipment.


Asunto(s)
Contaminantes Ocupacionales del Aire/toxicidad , Daño del ADN , Exposición Profesional/análisis , Plaguicidas/toxicidad , Adulto , Contaminantes Ocupacionales del Aire/metabolismo , Brasil , Ensayo Cometa , Femenino , Humanos , Masculino , Pruebas de Micronúcleos , Persona de Mediana Edad , Exposición Profesional/estadística & datos numéricos , Plaguicidas/metabolismo
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