RESUMEN
CONTEXT: Angelica sinensis L. (Umbelliferae) has medicinal properties. OBJECTIVES: The present study evaluates the haematopoietic effects of A. sinensis polysaccharides (ASP) against lisinopril-induced anaemia. MATERIALS AND METHODS: Thirty healthy adult male albino rats were randomly divided into five groups (n = 6). Group I was control group. Group II was treated with angiotensin-converting enzyme inhibitor (ACEI, 20 mg/kg/day) to induce anaemia. In group III, erythropoietin (EPO, 100 IU/kg/each) was administered in combination with ACEI. Group IV was treated with ASP (1 g/kg/day), extracted from A. sinensis root caps. In Group V, ASP (1 g/kg/day) was treated with ACEI. After 28 days, blood and tissue samples were collected for haematological and histopathological analysis, respectively. RESULTS: The results showed that ACEI significantly reduced the haemoglobin (Hb, 10.0 g/dL), packed cell volume (PCV, 39.5%), red blood cells (RBCs, 6.2 million/mm3), mean corpuscular volume (MCV, 53.5 fL) and mean corpuscular haemoglobin (MCH, 16.2 pg/cell) values. In the group treated with ASP, the Hb (13.7 g/dL) and RBCs (7.8 million/mm3) increased significantly (p < 0.05). The combination of ASP and ACEI led to the significant (p < 0.05) reduction in Hb (10.7 g/dL), PCV (33.3%), RBCs (6.0 million/mm3), MCV (54.42 fL) and MCH (16.44 pg/cell) values. While histopathological examination of the liver and kidney cells showed a mild degree of toxicity in the ASP-treated group. CONCLUSION: ASP has a potentiating effect on haematological parameters when given alone. However, when administered simultaneously with lisinopril, it showed an unfavourable effect with more complicated anaemia so it should not be used with ACEIs.
Asunto(s)
Anemia/tratamiento farmacológico , Angelica sinensis/química , Eritrocitos/efectos de los fármacos , Hematínicos/farmacología , Hematopoyesis/efectos de los fármacos , Lisinopril , Extractos Vegetales/farmacología , Cápsula de Raíz de Planta/química , Polisacáridos/farmacología , Anemia/sangre , Anemia/inducido químicamente , Animales , Biomarcadores/sangre , Modelos Animales de Enfermedad , Índices de Eritrocitos , Eritrocitos/metabolismo , Eritropoyetina/farmacología , Hematínicos/aislamiento & purificación , Hematínicos/toxicidad , Hematócrito , Hemoglobinas/metabolismo , Interacciones de Hierba-Droga , Masculino , Fitoterapia , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/toxicidad , Plantas Medicinales , Polisacáridos/aislamiento & purificación , Polisacáridos/toxicidad , Ratas Wistar , Factores de TiempoRESUMEN
Plant roots are able to sense soil nutrient availability. In order to acquire heterogeneously distributed water and minerals, they optimize their root architecture. One poorly understood plant response to soil phosphate (P(i)) deficiency is a reduction in primary root growth with an increase in the number and length of lateral roots. Here we show that physical contact of the Arabidopsis thaliana primary root tip with low-P(i) medium is necessary and sufficient to arrest root growth. We further show that loss-of-function mutations in Low Phosphate Root1 (LPR1) and its close paralog LPR2 strongly reduce this inhibition. LPR1 was previously mapped as a major quantitative trait locus (QTL); the molecular origin of this QTL is explained by the differential allelic expression of LPR1 in the root cap. These results provide strong evidence for the involvement of the root cap in sensing nutrient deficiency, responding to it, or both. LPR1 and LPR2 encode multicopper oxidases (MCOs), highlighting the essential role of MCOs for plant development.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Oxidorreductasas/metabolismo , Fosfatos/metabolismo , Raíces de Plantas/crecimiento & desarrollo , Secuencia de Aminoácidos , Arabidopsis/crecimiento & desarrollo , Proteínas de Arabidopsis/metabolismo , Mapeo Cromosómico , Cromosomas de las Plantas , Clonación Molecular , Cobre/química , Regulación de la Expresión Génica de las Plantas , Datos de Secuencia Molecular , Mutación/genética , Oxidorreductasas/genética , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/metabolismo , Raíces de Plantas/genética , Sitios de Carácter Cuantitativo , ARN Mensajero/genética , ARN Mensajero/metabolismo , ARN de Planta/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Aminoácido , Suelo/análisisRESUMEN
To defend themselves against herbivores and pathogens, plants produce numerous secondary metabolites, either constitutively or de novo in response to attacks. An intriguing constitutive example is the exudate produced by certain root-cap cells that can induce a state of reversible quiescence in plant-parasitic nematodes, thereby providing protection against these antagonists. The effect of such root exudates on beneficial entomopathogenic nematodes (EPNs) remains unclear, but could potentially impair their use in pest management programmes. We therefore tested how the exudates secreted by green pea (Pisum sativum) root caps affect four commercial EPN species. The exudates induced reversible quiescence in all EPN species tested. Quiescence levels varied with the green pea cultivars tested. Notably, after storage in root exudate, EPN performance traits were maintained over time, whereas performances of EPNs stored in water rapidly declined. In sharp contrast to high concentrations, lower concentrations of the exudate resulted in a significant increase in EPN activity and infectiousness, but still reduced the activity of two plant-parasitic nematode species. Our study suggests a finely tuned dual bioactivity of the exudate from green pea root caps. Appropriately formulated, it can favour long-term storage of EPNs and boost their infectiousness, while it may also be used to protect plants from plant-parasitic nematodes.
Asunto(s)
Interacciones Huésped-Parásitos/efectos de los fármacos , Nematodos/fisiología , Exudados de Plantas/farmacología , Cápsula de Raíz de Planta/química , Animales , Nematodos/efectos de los fármacos , Pisum sativum/química , Temperatura , Factores de TiempoRESUMEN
Maize (Zea mays L.) root cap cells secrete a large variety of compounds including proteins via an amorphous gel structure called mucilage into the rhizosphere. In the present study, mucilage secreted by primary roots of 3-4 day old maize seedlings was collected under axenic conditions, and the constitutively secreted proteome was analyzed. A total of 2848 distinct extracellular proteins were identified by nanoLC-MS/MS. Among those, metabolic proteins (approximately 25%) represented the largest class of annotated proteins. Comprehensive sets of proteins involved in cell wall metabolism, scavenging of reactive oxygen species, stress response, or nutrient acquisition provided detailed insights in functions required at the root-soil interface. For 85-94% of the mucilage proteins previously identified in the relatively small data sets of the dicot species pea, Arabidopsis, and rapeseed, a close homologue was identified in the mucilage proteome of the monocot model plant maize, suggesting a considerable degree of conservation between mono and dicot mucilage proteomes. Homologues of a core set of 12 maize proteins including three superoxide dismutases and four chitinases, which provide protection from fungal infections, were present in all three mucilage proteomes investigated thus far in the dicot species Arabidopsis, rapeseed, and pea and might therefore be of particular importance.
Asunto(s)
Mapeo Peptídico/métodos , Proteínas de Plantas/química , Cápsula de Raíz de Planta/química , Proteoma/química , Zea mays/química , Redes y Vías Metabólicas , Proteínas de Plantas/clasificación , Plantas , Homología Estructural de Proteína , Zea mays/metabolismoRESUMEN
Plant roots secrete a complex polysaccharide mucilage that may provide a significant source of carbon for microbes that colonize the rhizosphere. High molecular weight mucilage was separated by high-pressure liquid chromatography gel filtration from low molecular weight components of pea root exudate. Purified pea root mucilage generally was similar in sugar and glycosidic linkage composition to mucilage from cowpea, wheat, rice, and maize, but appeared to contain an unusually high amount of material that was similar to arabinogalactan protein. Purified pea mucilage was used as the sole carbon source for growth of several pea rhizosphere bacteria, including Rhizobium leguminosarum 8401 and 4292, Burkholderia cepacia AMMD, and Pseudomonas fluorescens PRA25. These species grew on mucilage to cell densities of three- to 25-fold higher than controls with no added carbon source, with cell densities of 1 to 15% of those obtained on an equal weight of glucose. Micromolar concentrations of nod gene-inducing flavonoids specifically stimulated mucilage-dependent growth of R. leguminosarum 8401 to levels almost equaling the glucose controls. R. leguminosarum 8401 was able to hydrolyze p-nitrophenyl glycosides of various sugars and partially utilize a number of purified plant polysaccharides as sole carbon sources, indicating that R. leguminosarum 8401 can make an unexpected variety of carbohydrases, in accordance with its ability to extensively utilize pea root mucilage.
Asunto(s)
Burkholderia cepacia/metabolismo , Carbono/metabolismo , Pisum sativum/microbiología , Proteoglicanos/metabolismo , Pseudomonas fluorescens/metabolismo , Rhizobium leguminosarum/metabolismo , Aminoácidos/química , Aminoácidos/metabolismo , Burkholderia cepacia/crecimiento & desarrollo , Metabolismo de los Hidratos de Carbono , Carbohidratos/química , Flavonoides/farmacología , Pisum sativum/metabolismo , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/metabolismo , Cápsula de Raíz de Planta/microbiología , Proteoglicanos/química , Pseudomonas fluorescens/crecimiento & desarrollo , Rhizobium leguminosarum/crecimiento & desarrollo , SimbiosisRESUMEN
NASA: Defining interactions of roots with the surrounding soil environment has been the focus of many recent investigations. As a result of these efforts, we are gaining an appreciation of the varied and often surprising strategies whereby roots adjust to and condition their soil environment for optimal growth and development. This article summarizes current knowledge of the often complex interactions between roots and biotic and abiotic factors within the soil. These interactions are interpreted in terms of modifications in the development or the physiology of the root.^ieng
Asunto(s)
Cápsula de Raíz de Planta/crecimiento & desarrollo , Cápsula de Raíz de Planta/fisiología , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/fisiología , Hongos/fisiología , Gravitropismo/fisiología , Nitrógeno/metabolismo , Fijación del Nitrógeno , Cápsula de Raíz de Planta/química , Raíces de Plantas/metabolismo , Rhizobium/fisiología , Suelo/análisis , Microbiología del Suelo , Simbiosis , AguaRESUMEN
Light is essential for root gravitropism in Zea mays L., cultivar Merit. It is hypothesized that calcium mediates this light-regulated response. KN-93, an inhibitor of calcium/calmodulin kinase II (CaMK II), inhibits light-regulated root gravitropism but does not affect light perception. We hypothesize that CaMK II, or a homologue, operates late in the light/gravity signal transduction chain. Here we provide evidence suggesting a possible physiological involvement of CaMK II in root gravitropism in plants.
Asunto(s)
Bencilaminas/farmacología , Proteínas Quinasas Dependientes de Calcio-Calmodulina/fisiología , Calcio/fisiología , Gravitropismo/fisiología , Luz , Raíces de Plantas/fisiología , Sulfonamidas/farmacología , Bencilaminas/metabolismo , Proteínas Quinasas Dependientes de Calcio-Calmodulina/antagonistas & inhibidores , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Inhibidores Enzimáticos , Gravitropismo/efectos de los fármacos , Fosforilación , Fototropismo/efectos de los fármacos , Fototropismo/fisiología , Proteínas de Plantas/análisis , Proteínas de Plantas/metabolismo , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/efectos de los fármacos , Cápsula de Raíz de Planta/fisiología , Raíces de Plantas/química , Raíces de Plantas/efectos de los fármacos , Inhibidores de Proteínas Quinasas , Proteínas Quinasas/metabolismo , Proteínas Quinasas/fisiología , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Sulfonamidas/metabolismo , Factores de Tiempo , Zea mays/química , Zea mays/efectos de los fármacos , Zea mays/fisiologíaRESUMEN
Root tips of intact willow (Salix dasyclados Wimm., Clone 81-090) plants were partially dried by exposure to ambient greenhouse air and then kept in water-vapor-saturated air for up to 3 days. The drying treatment increased abscisic acid (ABA) concentrations in both the root tips subjected to drying and in the xylem sap, while it reduced leaf stomatal conductance and leaf extension rate. Despite the decrease in stomatal conductance, leaf water potentials were unaffected by the root drying treatment, indicating that the treatment reduced hydraulic conductivity between roots and foliage. After roots subjected to drying were returned to a nutrient solution or excised, ABA concentrations in the remaining roots and in the xylem sap, stomatal conductance of mature leaves and extension rate of unfolding leaves all returned to values observed in control plants. The 4-fold increase in xylem sap ABA concentration following the root drying treatment was not solely the result of reduced sap flow, and thus may be considered a potential cause, not merely a consequence, of the observed reduction in stomatal conductance.
Asunto(s)
Hojas de la Planta/crecimiento & desarrollo , Cápsula de Raíz de Planta/fisiología , Salicaceae/fisiología , Árboles/fisiología , Ácido Abscísico/análisis , Hojas de la Planta/química , Cápsula de Raíz de Planta/química , Tallos de la Planta/fisiología , Transpiración de Plantas/fisiología , Agua/fisiologíaRESUMEN
Aluminum (Al) tolerance mechanisms in crop plants have been extensively researched, but our understanding of the physiological mechanisms underlying Al tolerance in trees is still limited. To investigate Al tolerance in eucalypts, seedlings of six species (Eucalyptus globulus Labill., Eucalyptus urophylla S.T. Blake, Eucalyptus dunnii Maiden, Eucalyptus saligna Sm., Eucalyptus cloeziana F. J. Muell. and Eucalyptus grandis w. Hill ex Maiden) and seedlings of six clones of Eucalyptus species were grown for 10 days in nutrient solutions containing Al concentrations varying from 0 to 2.5 microM (0 to 648 microM Al3+ activities). Root elongation of most species was inhibited only by high Al3+ activities. Low to intermediate Al3+ activities were beneficial to root elongation of all species and clones. Among the species tested, E. globulus and E. urophylla were more tolerant to Al toxicity, whereas E. grandis and E. cloeziana were more susceptible to Al-induced damage. Although E. globulus seedlings were tolerant to Al toxicity, they were highly sensitive to lanthanum (La), indicating that the tolerance mechanism is specific for Al. Fine roots accumulated more Al and their elongation was inhibited more than that of thick roots. In E. globulus, accumulation of Al in root tips increased linearly with increasing Al concentration in the nutrient solution. The majority of Al taken up was retained in the root system, and the small amounts of Al translocated to the shoot system were found mainly in older leaves. No more than 60% of the Al in the thick root tip was in an exchangeable form in the apoplast that could be removed by sequential citrate rinses. Gas chromatography/mass spectrometry and ion chromatography analyses indicated that root exposure to Al led to a greater than 200% increase in malic acid concentration in the root tips of all eucalypt species. The increase in malate concentration in response to Al treatment correlated with the degree of Al tolerance of the species. A small increase in citric acid concentration was also observed in all species, but there were no consistent changes in the concentrations of other organic acids in response to Al treatment. In all eucalypt species, Al treatment induced the secretion of citric and malic acid in root exudates, but no trend with respect to Al tolerance was observed. Thus, although malate and citrate exudation by roots may partially account for the overall high Al tolerance of these eucalypt species, it appears that tolerance is mainly derived from the internal detoxification of Al by complexation with malic acid.
Asunto(s)
Aluminio/farmacología , Eucalyptus/efectos de los fármacos , Árboles/efectos de los fármacos , Aluminio/análisis , Ácido Cítrico/metabolismo , Eucalyptus/fisiología , Cápsula de Raíz de Planta/química , Raíces de Plantas/química , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/fisiología , Brotes de la Planta/química , Árboles/fisiologíaRESUMEN
The object of this research was to determine how effectively the actions of a clinostat and a fluid-filled, slow-turning lateral vessel (STLV) mimic the ultrastructural effects of microgravity in plant cells. We accomplished this by qualitatively and quantitatively comparing the ultrastructures of cells grown on clinostats and in an STLV with those of cells grown at 1 g and in microgravity aboard the Space Shuttle Columbia. Columella cells of Brassica perviridis seedlings grown in microgravity and in an STLV have similar structures. Both contain significantly more lipid bodies, less starch, and fewer dictyosomes than columella cells of seedlings grown at 1 g. Cells of seedlings grown on clinostats have significantly different ultrastructures from those grown in microgravity or in an STLV, indicating that clinostats do not mimic microgravity at the ultrastructural level. The similar structures of columella cells of seedlings grown in an STLV and in microgravity suggest that an STLV effectively mimics microgravity at the ultrastructural level.
Asunto(s)
Brassica/ultraestructura , Cápsula de Raíz de Planta/ultraestructura , Vuelo Espacial/instrumentación , Simulación de Ingravidez/métodos , Ingravidez , Brassica/química , Brassica/citología , Gravitación , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/citología , Plastidios/ultraestructura , Rotación , Almidón/análisisRESUMEN
Antimonate staining procedures and energy dispersive X-ray micro-analytical techniques were used to determine the patterns of localization of calcium in nonstimulated and gravistimulated corn roots. In horizontally positioned roots within the region of the developing bend there was a change in the staining from that principally localized within cells of the stele to asymmetric staining within the vacuoles of the cortical cells along the upper root surface. There was little staining in the walls. The pattern observed is quite different from that seen in gravistimulated coleoptiles. Staining of mitochondria, plastids and Golgi stacks was seen in most cell types, but no asymmetry of staining was observed. In the rootcap where graviperception is thought to occur, there was little staining of any cellular organelles.
Asunto(s)
Calcio/análisis , Gravitropismo/fisiología , Raíces de Plantas/química , Raíces de Plantas/ultraestructura , Zea mays/química , Antimonio , Gravitación , Microscopía Electrónica , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/crecimiento & desarrollo , Cápsula de Raíz de Planta/ultraestructura , Raíces de Plantas/crecimiento & desarrollo , Análisis Espectral , Coloración y Etiquetado , Vacuolas/química , Vacuolas/ultraestructura , Zea mays/crecimiento & desarrollo , Zea mays/ultraestructuraRESUMEN
Ca2+ is implicated as a messenger in coupling various environmental stimuli, such as gravity and light, to response. In recent years, it has become evident that Ca2+ plays a central role in all three phases of gravitropism--perception, transduction and response. The root cap, which is known to contain high amounts of Ca2+ and calmodulin, is the primary site of gravity perception. The possible role of phosphoinositide turnover and Ca(2+) - and Ca(2+) -calmodulin-dependent enzymes such as Ca(2+) -ATPase and protein kinases in gravitropism is discussed. A model is proposed to describe the role of Ca2+ in both normal and light-dependent gravity response in roots.
Asunto(s)
Calcio/fisiología , Gravitropismo/fisiología , Sensación de Gravedad/fisiología , Fenómenos Fisiológicos de las Plantas , Transducción de Señal/fisiología , Calcio/análisis , Canales de Calcio/fisiología , ATPasas Transportadoras de Calcio/fisiología , Calmodulina/análisis , Calmodulina/fisiología , Luz , Desarrollo de la Planta , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/crecimiento & desarrollo , Cápsula de Raíz de Planta/fisiología , Plantas/químicaRESUMEN
Maize (Zea mays) roots respond to a variety of environmental stimuli which are perceived by a specialized group of cells, the root cap. We are studying the transduction of extracellular signals by roots, particularly the role of protein kinases. Protein phosphorylation by kinases is an important step in many eukaryotic signal transduction pathways. As a first phase of this research we have isolated a cDNA encoding a maize protein similar to fungal and animal protein kinases known to be involved in the transduction of extracellular signals. The deduced sequence of this cDNA encodes a polypeptide containing amino acids corresponding to 33 out of 34 invariant or nearly invariant sequence features characteristic of protein kinase catalytic domains. The maize cDNA gene product is more closely related to the branch of serine/threonine protein kinase catalytic domains composed of the cyclic-nucleotide- and calcium-phospholipid-dependent subfamilies than to other protein kinases. Sequence identity is 35% or more between the deduced maize polypeptide and all members of this branch. The high structural similarity strongly suggests that catalytic activity of the encoded maize protein kinase may be regulated by second messengers, like that of all members of this branch whose regulation has been characterized. Northern hybridization with the maize cDNA clone shows a single 2400 base transcript at roughly similar levels in maize coleoptiles, root meristems, and the zone of root elongation, but the transcript is less abundant in mature leaves. In situ hybridization confirms the presence of the transcript in all regions of primary maize root tissue.
Asunto(s)
Cápsula de Raíz de Planta/química , Raíces de Plantas/fisiología , Proteínas Quinasas/fisiología , Transducción de Señal/fisiología , Zea mays/genética , Secuencia de Aminoácidos , ADN Complementario/aislamiento & purificación , Biblioteca Genómica , Datos de Secuencia Molecular , Péptidos/química , Péptidos/fisiología , Proteínas de Vegetales Comestibles/química , Cápsula de Raíz de Planta/genética , Cápsula de Raíz de Planta/fisiología , Raíces de Plantas/genética , Proteínas Quinasas/análisis , Proteínas Quinasas/genética , Sistemas de Mensajero Secundario , Transducción de Señal/genética , Zea mays/fisiologíaRESUMEN
It has been hypothesized that the sedimentation of amyloplasts within root cap cells is the primary event in the plant gravisensory-signal transduction cascade. Statolith sedimentation, with its ability to generate weighty mechanical signals, is a legitimate means for organisms to discriminate the direction of the gravity vector. However, it has been demonstrated that starchless mutants with reduced statolith densities maintain some ability to sense gravity, calling into question the statolith sedimentation hypothesis. Here we report on the presence of a beta 1 integrin-like protein localized inside amyloplasts of tobacco NT-1 suspension culture, callus cells, and whole-root caps. Two different antibodies to the beta 1 integrin, one to the cytoplasmic domain and one to the extracellular domain, localize in the vicinity of the starch grains within amyloplasts of NT-1. Biochemical data reveals a 110-kDa protein immunoprecipitated from membrane fractions of NT-1 suspension culture indicating size homology to known beta 1 integrin in animals. This study provides the first direct evidence for the possibility of integrin-mediated signal transduction in the perception of gravity by higher plants. An integrin-mediated pathway, initiated by starch grain sedimentation within the amyloplast, may provide the signal amplification necessary to explain the gravitropic response in starch-depleted cultivars.
Asunto(s)
Sensación de Gravedad/fisiología , Integrina beta1/análisis , Proteínas de Plantas/análisis , Plastidios/química , Transducción de Señal/fisiología , Integrinas/análisis , Microscopía Electrónica , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/citología , Cápsula de Raíz de Planta/fisiología , Cápsula de Raíz de Planta/ultraestructura , Plantas Tóxicas , Plastidios/fisiología , Plastidios/ultraestructura , Almidón , Nicotiana/química , Nicotiana/citología , Nicotiana/fisiología , Nicotiana/ultraestructuraRESUMEN
In certain cultivars of corn (Zea mays var. Merit), light stimulates gravitropic bending of the root by influencing events in the root cap. In this paper, we report on changes in root cap proteins which occur as a result of the light treatment and single out specific proteins as potentially having a role in mediating the gravitropic response. For this work, we have used root caps maintained aseptically in culture media supplemented with auxin. If auxin is deleted from the culture medium, the protein profiles observed following illumination differ from that seen in caps provided light while in auxin-supplemented media. We also report that several of the proteins for which synthesis is stimulated by light appear to turn over rapidly, usually within 0.5 hour of formation.
Asunto(s)
Gravitropismo/fisiología , Luz , Proteínas de Plantas/metabolismo , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/metabolismo , Zea mays/crecimiento & desarrollo , Gravitropismo/efectos de la radiación , Ácidos Indolacéticos/farmacología , Proteínas de Plantas/análisis , Proteínas de Plantas/biosíntesis , Cápsula de Raíz de Planta/efectos de los fármacos , Cápsula de Raíz de Planta/crecimiento & desarrollo , Zea mays/química , Zea mays/efectos de los fármacos , Zea mays/metabolismoRESUMEN
In Phaseolus vulgaris, primary roots show gravitational sensitivity soon after emerging from the seed. In contrast, lateral roots are agravitropic during early development, and become gravitropic after several cm growth. Primary and lateral root tissues were examined by polyacrylamide gel electrophoresis, coupled with western blotting techniques, to compare proteins which may contribute to the acquisition of gravitational sensitivity. Root tips and zones of cell elongation were compared for each root type, using immunological probes for calmodulin, alpha-actin, alpha-tubulin, and proteins of the plastid envelope. Lateral roots contained qualitatively less calmodulin, and showed a slightly different pattern of actin-related epitope proteins, than did primary root tissues, suggesting that polypeptide differences may contribute to the gravitational sensitivity which these root types express.
Asunto(s)
Fabaceae/fisiología , Sensación de Gravedad/fisiología , Péptidos/análisis , Proteínas de Plantas/análisis , Raíces de Plantas/química , Plantas Medicinales , Actinas/análisis , Calmodulina/análisis , Fabaceae/química , Fabaceae/crecimiento & desarrollo , Gravitropismo/fisiología , Péptidos/fisiología , Proteínas de Plantas/fisiología , Cápsula de Raíz de Planta/química , Cápsula de Raíz de Planta/citología , Cápsula de Raíz de Planta/fisiología , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/fisiología , Plastidios/química , Plastidios/fisiología , Tubulina (Proteína)/análisisRESUMEN
Using a polyclonal antibody raised against calreticulin purified and sequenced from maize, we performed an immunocytological study to characterize putative domain-specific subcellular distributions of endoplasmic reticulum (ER)-resident calreticulin in meristematic cells of maize root tip. At the light microscopy level, calreticulin was immunolocalized preferentially at cellular peripheries, in addition to nuclear envelopes and cytoplasmic structures. Punctate labelling at the longitudinal walls and continuous labelling at the transverse walls was characteristic. Immunogold electron microscopy revealed plasmodesmata as the most prominently labelled cell periphery structure. In order to further probe the ER-domain-specific distribution of maize calreticulin at plasmodesmata, root apices were exposed to mannitol-induced osmotic stress. Plasmolysis was associated with prominent accumulations of calreticulin at callose-enriched plasmodesmata and pit fields while the contracting protoplasts were depleted of calreticulin. In contrast, other ER-resident proteins recognized by HDEL peptide and BiP antibodies localized exclusively to contracted protoplasts. This finding reveals that, in plasmolysed cells, calreticulin enriched ER domains at plasmodesmata and pit fields are depleted of other ER-resident proteins containing the HDEL retention peptide.