RESUMEN
STUDY OBJECTIVE: To assess the safety, tolerability and pharmacokinetics of escalating single doses of wood creosote, an herbal antidiarrheal and antispasmodic agent. DESIGN: Randomized, double-blind, placebo-controlled study. SETTING: Clinical research center. SUBJECTS: Forty (32 men, 8 women) healthy volunteers aged 19-42 years. INTERVENTION: By random assignment, 22 men and 8 women received escalating single doses of wood creosote (45, 90, 135, 180, and 225 mg) and 10 men received placebo (for each of the five dose levels, 6 subjects received active substance and 2 subjects received placebo). MEASUREMENTS AND MAIN RESULTS: Vital signs, laboratory tests, and electrocardiograms were assessed; no dose-related or clinically significant changes were noted. Serial blood samples were obtained to determine the pharmacokinetics of four major active components of wood creosote: total (conjugated plus free) guaiacol, creosol, o-cresol, and 4-ethylguaiacol. The most common adverse events were mild headache and dizziness, with no dose-related trends being apparent. Area under the concentration-time curve from time zero to infinity increased in a dose-proportional manner for total guaiacol, creosol, and o-cresol and was not assessed for total 4-ethylguaiacol owing to lack of data at the low dose level. No apparent differences by sex were noted for any of the four active components. All four components were rapidly eliminated. CONCLUSION: Single oral doses of wood creosote up to 225 mg were safe and well tolerated in healthy men and women. Also, the doses of wood creosote were rapidly absorbed, conjugated, and eliminated. Such a rapid onset and short duration of action would appear desirable in the treatment of acute nonspecific diarrhea.
Asunto(s)
Antidiarreicos/farmacocinética , Creosota/farmacocinética , Extractos Vegetales/farmacocinética , Preparaciones de Plantas/farmacocinética , Madera , Adulto , Antidiarreicos/administración & dosificación , Antidiarreicos/efectos adversos , Área Bajo la Curva , Presión Sanguínea/efectos de los fármacos , Presión Sanguínea/fisiología , Creosota/administración & dosificación , Creosota/efectos adversos , Relación Dosis-Respuesta a Droga , Método Doble Ciego , Esquema de Medicación , Femenino , Frecuencia Cardíaca/efectos de los fármacos , Frecuencia Cardíaca/fisiología , Humanos , Modelos Lineales , Masculino , Extractos Vegetales/administración & dosificación , Extractos Vegetales/efectos adversos , Preparaciones de Plantas/administración & dosificación , Preparaciones de Plantas/efectos adversosRESUMEN
Quantification of risks to the ecosystem is necessary for cost-effective remediation strategies. Contaminant endpoints need to be established that consider the bioavailability of toxicants in soil. The challenge is to develop methods that assign risk to the bioavailable toxic contaminants, thereby protecting ecosystems, while balancing remediation costs. Our objective was to evaluate changes in bioavailability of creosote constituents in soils to earthworms. An acute ecotoxicological investigation of three weathered creosote-contaminated and two slurry-phase-biotreated soils was conducted using a 14-d earthworm (Eisenia fetida) survival bioassay. Soil characterization (physical and chemical) and contaminant concentration data (polycyclic aromatic hydrocarbons [PAH] and total dichloromethane extractable organics [DEO]) were also determined. The toxicity of the soils could not always be predicted based on chemical concentrations alone. Soils having a low PAH:DEO ratio had higher cumulative earthworm survival times as measured by earthworm-days. We propose that the DEO fraction may regulate toxicity by altering bioavailability of toxicants.
Asunto(s)
Creosota/toxicidad , Oligoquetos , Contaminantes del Suelo/toxicidad , Animales , Disponibilidad Biológica , Creosota/farmacocinética , Predicción , Dosificación Letal Mediana , Medición de Riesgo , Contaminantes del Suelo/farmacocinéticaRESUMEN
A thirty-eight year old man took about 180 tablets of Seirogan. He was unconscious and had dyspnea with dark brown urine on admission. He recovered gradually after initial treatment. Seirogan contains a phenolic component. Symptoms and signs of poisoning are unconsciousness, convulsion, digestive tract disorder, pulmonary edema, hepatic failure, renal failure, and miosis. Clinical features include dark brown urine. On day 7, he again showed signs of creosote poisoning: relapse of unconsciousness and dark colored urine. Plasma concentration of phenol determined on the day before the relapse was much higher than that expected from the half-life of blood phenol. It is reported that Creosote poisoning results in a decrease in the intestinal peristalsis, or paralytic ileus. We would like to emphasize that a relapse of Creosote poisoning may occur due to possible delayed absorption of the Seirogan tablets.
Asunto(s)
Antidiarreicos/envenenamiento , Creosota/envenenamiento , Medicamentos sin Prescripción/envenenamiento , Enfermedad Aguda , Adulto , Antidiarreicos/química , Creosota/farmacocinética , Semivida , Humanos , Seudoobstrucción Intestinal/inducido químicamente , Seudoobstrucción Intestinal/fisiopatología , Masculino , Persona de Mediana Edad , Medicamentos sin Prescripción/química , Peristaltismo/efectos de los fármacos , Recurrencia , Comprimidos , Factores de TiempoRESUMEN
Wood creosote, principally a mixture of non-, alkyl- and/or alkoxy-substituted phenolic compounds, was orally administered to adult male volunteers to determine its metabolites and pharmacokinetic parameters. After a 133-mg single dose, its major constituents (i.e. phenol 15 mg, guaiacol 32 mg, p-cresol 18 mg and creosol 24 mg) were found in peripheral venous blood and urine, mostly as glucuronic acid and, except for creosol, as sulfate conjugates. Low concentrations of unconjugated phenols were also detected. The metabolites in the serum started to increase 15 min after the dose, and they reached their maximum concentrations 30 min after administration. The maximum concentrations of glucuronides were 0.18 +/- 0.07, 0.91 +/- 0.38, 0.33 +/- 0.18 and 0.47 +/- 0.23 mg/l; those of sulfates were 0.16 +/- 0.06, 0.22 +/- 0.09, 0.17 +/- 0.07 and < 0.04 mg/l for phenol, guaiacol, p-cresol and creosol, respectively. The 24-hour urinary recoveries of the sum of each compound and its metabolites were 75 +/- 35, 45 +/- 36, 103 +/- 51 and 74 +/- 36%, in the above order. The presence of guaiacol glucuronide in blood and urine was directly verified by its isolation and structure analyses.
Asunto(s)
Creosota/farmacocinética , Fenoles/farmacocinética , Madera , Adulto , Biotransformación , Cromatografía Líquida de Alta Presión , Cresoles/farmacocinética , Glucuronatos/metabolismo , Guayacol/farmacocinética , Humanos , Espectroscopía de Resonancia Magnética , Masculino , Sulfatos/metabolismoRESUMEN
OBJECTIVES: To evaluate workers' exposure in a creosote impregnation plant by means of ambient and biological monitoring. METHODS: Naphthalene (vapour phase) and 10 large molecular polycyclic aromatic hydrocarbons (PAHs) (particulate phase) were measured in the breathing zone air during an entire working week. 1-Hydroxypyrene (1-HP) was measured in 24 hour urine as a metabolite of the pyrene found in neat (dermal exposure) and airborne creosote. RESULTS: Naphthalene (0.4-4.2 mg/m3) showed 1000 times higher concentrations in air than did the particulate PAHs. In total, the geometric mean (range) of three to six ring PAHs was 4.8 (1.2-13.7) micrograms/m3; pyrene 0.86 (0.23-2.1) micrograms/m3, and benzo(a)pyrene 0.012 (0.01-0.05) micrograms/m3. There was no correlation between pyrene and gaseous naphthalene. The correlations between pyrene and the other nine particulate PAHs were strong, and gave a PAH profile that was similar in all air samples: r = 0.83 (three to six ring PAHs); r = 0.81 (three ring PAHs); r = 0.78 (four to six ring PAHs). Dermal exposure was probably very high in all workers, because the daily output of urinary 1-HP exceeded the daily uptake of inhaled pyrene by < or = 50-fold. Urinary 1-HP concentrations were very high, even on Monday mornings, when they were at their lowest (4-22 mumol/mol creatinine). 1-HP seldom showed any net increase over a workshift (except on Monday) due to its high concentrations (16 to 120 mumol/mol creatinine) in the morning samples. 1-HP was always lower at the end of the shift (19 to 85 mumol/mol creatinine) than in the evening (27 to 122), and the mean (SD) change over the working week (47 (18)) was greater than the change over Monday (35 (32)). The timing of 1-HP sampling is therefore very important. CONCLUSIONS: Urinary 1-HP proved to be a good biomarker of exposure to three to six ring PAHs but not to airborne naphthalene. Hence, biomonitoring based on 1-HP has to be completed with exposure assessment for naphthalene as a marker for creosote volatiles that mainly enter the body through the lungs.
Asunto(s)
Contaminantes Ocupacionales del Aire/análisis , Creosota/farmacocinética , Mutágenos/análisis , Exposición Profesional , Compuestos Policíclicos/análisis , Pirenos/análisis , Contaminantes Ocupacionales del Aire/farmacocinética , Monitoreo del Ambiente , Humanos , Pulmón/metabolismo , Masculino , Naftalenos/análisis , Compuestos Policíclicos/farmacocinética , Absorción CutáneaRESUMEN
AIMS: Two common reasons to explain slow environmental biodegradation of polycyclic aromatic hydrocarbons (PAHs), namely lack of appropriate carbon sources for microbial growth and limited bioavailability of PAHs, were tested in a laboratory bioassay using a creosote-contaminated soil. METHODS AND RESULTS: The soil, containing a total of 8 mg g-1 of 16 PAHs, was sieved and incubated in bottles for 45 days. The first explanation was tested by enrichment with the analogue anthracene and the non-analogue myristic acid, and both failed to stimulate degradation of all PAHs except anthracene. The second explanation was tested by addition of different concentrations of dissolved organic carbon (DOC), with effects depending on the DOC concentration and the molecular size of the PAH. The degradation was enhanced from 10 to 35% for 12 PAHs when the soil was saturated. The degraded amounts of individual PAHs were proportional to their concentration in the soil. CONCLUSIONS: The slow in situ degradation of PAHs was enhanced by more than three times by adding water as a solvent. Addition of DOC facilitated the degradation of four- to six-ring PAHs. SIGNIFICANCE AND IMPACT OF STUDY: Bioremediation of PAH-contaminated sites may be facilitated by creating water-saturated conditions but retarded by addition of other carbon substrates, such as analogue compounds.