H2-DMalpha(-/-) mice show the importance of major histocompatibility complex-bound peptide in cardiac allograft rejection.
J Exp Med
; 192(1): 31-40, 2000 Jul 03.
Article
in En
| MEDLINE
| ID: mdl-10880524
ABSTRACT
The role played by antigenic peptides bound to major histocompatibility complex (MHC) molecules is evaluated with H2-DMalpha(-/)- mice. These mice have predominantly class II-associated invariant chain peptide (CLIP)-, not antigenic peptide-bound, MHC class II. H2-DMalpha(-/)- donor heart grafts survived three times longer than wild-type grafts and slightly longer than I-A(beta)(b)-(/)- grafts. Proliferative T cell response was absent, and cytolytic response was reduced against the H2-DMalpha(-/)- grafts in vivo. Residual cytolytic T cell and antibody responses against intact MHC class I lead to eventual rejection. Removal of both H2-DMalpha and beta2-microglobulin (beta2m) in cardiac grafts lead to greater (8-10 times) graft survival, whereas removal of beta2m alone did not have any effect. These results demonstrate the significance of peptide rather than just allogeneic MHC, in eliciting graft rejection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
HLA-D Antigens
/
Heart Transplantation
/
Th2 Cells
/
Th1 Cells
/
Graft Rejection
/
Major Histocompatibility Complex
Limits:
Animals
Language:
En
Journal:
J Exp Med
Year:
2000
Type:
Article
Affiliation country:
United States