Specific PCR identification of the T1 vaccine strains for contagious bovine pleuropneumonia.
Mol Cell Probes
; 14(4): 205-10, 2000 Aug.
Article
in En
| MEDLINE
| ID: mdl-10970724
ABSTRACT
A specific PCR test for the identification of the vaccine strains T1, T1/44 and T1sr, was developed. This PCR reaction is based on variations of DNA sequences in a region flanking one IS1296 copy. The specific primer pair MmmSCP1-T1M2 amplifies a 700-bp long DNA fragment in the T1 vaccine strains and gives no amplification with the 60 other Mycoplasma mycoides subsp. mycoides SC strains tested. This PCR will permit to distinguish the T1 strain from all other vaccine strains and therefore avoid possible confusions. In addition, it should enable better investigations of post-vaccinal reactions.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pleuropneumonia, Contagious
/
Cattle Diseases
/
Polymerase Chain Reaction
/
Mycoplasma mycoides
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
Africa
Language:
En
Journal:
Mol Cell Probes
Journal subject:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
Year:
2000
Type:
Article
Affiliation country:
France