Cleavage of non-tRNA substrates by eukaryal tRNA splicing endonucleases.
EMBO Rep
; 2(3): 217-21, 2001 Mar.
Article
in En
| MEDLINE
| ID: mdl-11266363
ABSTRACT
Eukaryal tRNA splicing endonucleases use the mature domains of pre-tRNAs as their primary recognition elements. However, they can also cleave in a mode that is independent of the mature domain, when substrates are able to form the bulge-helix-bulge structure (BHB), which is cleaved by archaeal tRNA endonucleases. We present evidence that the eukaryal enzymes cleave their substrates after forming a structure that resembles the BHB. Consequently, these enzymes can cleave substrates that lack the mature domain altogether. That raises the possibility that these enzymes could also cleave non-tRNA substrates that already have a BHB. As predicted, they can do so, both in vitro and in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA Splicing
/
Endoribonucleases
Limits:
Animals
Language:
En
Journal:
EMBO Rep
Journal subject:
BIOLOGIA MOLECULAR
Year:
2001
Type:
Article
Affiliation country:
Italy