Shear stress modulates tumour cell adhesion to the endothelium.
Biorheology
; 40(1-3): 41-5, 2003.
Article
in En
| MEDLINE
| ID: mdl-12454385
ABSTRACT
The adhesion of breast adenocarcinoma cells (MDA-MB-231) to human umbilical vein endothelial cells (HUVEC) was studied in whole blood and under varying flow conditions. This study was done on HUVEC either kept under static conditions or pre-conditioned in flow for 2 hours at a shear stress of 5 or 13 dyn/cm(2). Coverslips coated by HUVEC were placed in a parallel plate perfusion chamber and perfused at a shear rate of 300 or 1500 sec(-1) with heparin-anticoagulated blood containing 111In labelled MDA-MB-231 cells. We report here the optimal conditions for studying the adhesion of MDA-MB-231 to endothelial cells under shear constraints corresponding to those observed into small and medium sized arteries.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Breast Neoplasms
/
Endothelium, Vascular
/
Adenocarcinoma
/
Neoplastic Cells, Circulating
Limits:
Female
/
Humans
Language:
En
Journal:
Biorheology
Year:
2003
Type:
Article
Affiliation country:
France