Single-molecule detection technologies in miniaturized high-throughput screening: fluorescence intensity distribution analysis.
J Biomol Screen
; 8(1): 19-33, 2003 Feb.
Article
in En
| MEDLINE
| ID: mdl-12854995
ABSTRACT
Single-molecule detection technologies are becoming a powerful readout format to support ultra-high-throughput screening. These methods are based on the analysis of fluorescence intensity fluctuations detected from a small confocal volume element. The fluctuating signal contains information about the mass and brightness of the different species in a mixture. The authors demonstrate a number of applications of fluorescence intensity distribution analysis (FIDA), which discriminates molecules by their specific brightness. Examples for assays based on brightness changes induced by quenching/dequenching of fluorescence, fluorescence energy transfer, and multiple-binding stoichiometry are given for important drug targets such as kinases and proteases. FIDA also provides a powerful method to extract correct biological data in the presence of compound fluorescence.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microscopy, Confocal
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
En
Journal:
J Biomol Screen
Journal subject:
BIOLOGIA MOLECULAR
Year:
2003
Type:
Article
Affiliation country:
Germany