[Preliminary studies on the mechanisms of a new anti-tumor agent PH II-7 with special preference to multidrug resistant tumor cells].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
; 24(2): 134-9, 2002 Apr.
Article
in Zh
| MEDLINE
| ID: mdl-12905789
ABSTRACT
OBJECTIVE:
To determine the anti-tumor activity of PH II-7 in vitro and explore preliminarily its mechanisms.METHODS:
The anti-tumor activity was measured using colorimetric MTT assay. Apoptosis was determined with fluorescence-activated cell sorter (FACS), electron microscopy and agarose gel electrophoresis. The expressions of mdr1 and sorcin genes were determined by Northern blot assay.RESULTS:
PH II-7 inhibited the proliferation of various human tumor cells derived from different tumor cell lines. The IC50 values varied from 0.34-18.61 mumol/L. Especially, PH II-7 had strong inhibitory effect on multidrug resistant tumor cells, whereas adriamycin (ADR) was resistant. Apoptosis was induced in HL60 and HL60/ADR cells treated with 1 microgram/ml PH II-7, while PH II-7 inhibited the expressions of mdr1 and sorcin genes.CONCLUSIONS:
PH II-7 is a new potential agent which has strong inhibitory effect on both multidrug resistant cells and their parental cells. PH II-7 may increase the intracellular drug concentration in MDR cells by inhibiting the expressions of the MDR-related genes mdr1 and sorcin and induce the apoptosis of MDR cells and their parental tumor cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Drugs, Chinese Herbal
/
Apoptosis
/
Antineoplastic Agents, Phytogenic
Limits:
Humans
Language:
Zh
Journal:
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
Year:
2002
Type:
Article
Affiliation country:
China