Tertiary structure around the guanosine-binding site of the Tetrahymena ribozyme.
Science
; 256(5056): 526-9, 1992 Apr 24.
Article
in En
| MEDLINE
| ID: mdl-1315076
ABSTRACT
A cleavage reagent directed to the active site of the Tetrahymena catalytic RNA was synthesized by derivatization of the guanosine substrate with a metal chelator. When complexed with iron(II), this reagent cleaved the RNA in five regions. Cleavage at adenosine 207, which is far from the guanosine-binding site in the primary and secondary structure, provides a constraint for the higher order folding of the RNA. This cleavage site constitutes physical evidence for a key feature of the Michel-Westhof model. Targeting a reactive entity to a specific site should be generally useful for determining proximity within folded RNA molecules or ribonucleoprotein complexes.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tetrahymena
/
RNA, Catalytic
/
Guanosine
Limits:
Animals
Language:
En
Journal:
Science
Year:
1992
Type:
Article