Production and characterization of a murine/human chimeric anti-idiotype antibody that mimics ganglioside.

Hastings, A; Morrison, S L; Kanda, S; Saxton, R E; Irie, R F

Hastings, A; Morrison, S L; Kanda, S; Saxton, R E; Irie, R F.

Affiliation

Hastings A; Department of Surgical Oncology, School of Medicine, University of California, Los Angeles 90024.

Cancer Res ; 52(7): 1681-6, 1992 Apr 01.

Article in En | MEDLINE | ID: mdl-1348012

ABSTRACT

ABSTRACT

The VL and VH from a murine anti-idiotypic antibody that mimics ganglioside have been cloned, sequenced, and expressed as a chimeric mouse/human IgG1 antibody. The chimeric antibody retained a binding specificity indistinguishable from the original murine antibody. The VH was a member of Vgam 3.8 family. The sequences are discussed in terms of ways in which proteins may mimic ganglioside epitopes.

Subject(s)

Antibodies, Anti-Idiotypic/genetics; Gangliosides/immunology; Immunoglobulin Heavy Chains/genetics; Immunoglobulin Light Chains/genetics; Amino Acid Sequence; Animals; Antibodies, Anti-Idiotypic/immunology; Antibodies, Anti-Idiotypic/isolation & purification; Base Sequence; Cell Line; Chimera; Cloning, Molecular; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulin J-Chains/genetics; Mice; Molecular Sequence Data; Multiple Myeloma/immunology; Oligodeoxyribonucleotides; Poly A/genetics; Poly A/isolation & purification; Protein Sorting Signals/genetics; RNA, Messenger/genetics; RNA, Messenger/isolation & purification; Recombinant Proteins/immunology; Recombinant Proteins/isolation & purification; Transfection

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Collection: 01-internacional Database: MEDLINE Main subject: Antibodies, Anti-Idiotypic / Immunoglobulin Heavy Chains / Immunoglobulin Light Chains / Gangliosides Limits: Animals / Humans Language: En Journal: Cancer Res Year: 1992 Type: Article

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