Correlation of morphological findings with functional reserve in the aging donor: role of the poly (ADP-ribose) polymerase.
Transplant Proc
; 36(3): 733-5, 2004 Apr.
Article
in En
| MEDLINE
| ID: mdl-15110646
ABSTRACT
INTRODUCTION:
The enzyme poly (ADP-ribose) polymerase (PARP-1) participates in the first events of DNA repair in higher organisms. Under conditions of tissue ischemia, this action can lead to significant decreases in NAD(+), massive adenosine triphosphate (ATP) depletion, and cell death. In renal grafts with pretransplantation cold ischemia and subsequent ischemia-reperfusion injury, overactivation of PARP-1 may lead to a higher index of acute tubular necrosis, a delay in total recovery of the function of the transplanted organ, and an early progression to chronic graft nephropathy. The present study examined whether increased tubular expression of PARP-1 in kidneys from aged donors contributed to recipient renal function. MATERIAL ANDMETHOD:
We studied the nuclear expression of PARP-1 using immunohistochemistry with monoclonal antibody PAR01 in 75 kidney biopsy specimens from 40 aged donors.RESULTS:
Immunohistochemical expression of PARP-1 showed a statistically significant relationship with donor age (r =.408, P =.006, Spearman test), with time required to achieve effective diuresis (r =.386, P =.01, Spearman test) and with creatinine levels in the first 3 months. We also highlighted a greater intensity of PARP-1 expression in suboptimal donor kidneys that failed to reduce the serum creatinine levels to <1.7 mg/dL (creatinine <1.7 PARP 1.29 +/- 1.49 vs creatinine >1.7 PARP 2.29 +/- 1.33, P =.047, Mann-Whitney U test).CONCLUSION:
We conclude that the determination of PARP-1 in biopsy specimens from aged donors may be a useful predictive factor for renal graft function.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tissue Donors
/
Kidney Transplantation
/
Poly(ADP-ribose) Polymerases
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Aged
/
Humans
Language:
En
Journal:
Transplant Proc
Year:
2004
Type:
Article
Affiliation country:
Spain