Correlation between Helicobacter pylori OipA protein expression and oipA gene switch status.

Kudo, Takahiko; Nurgalieva, Zhannat Z; Conner, Margaret E; Crawford, Sue; Odenbreit, Stefan; Haas, Rainer; Graham, David Y; Yamaoka, Yoshio

Kudo, Takahiko; Nurgalieva, Zhannat Z; Conner, Margaret E; Crawford, Sue; Odenbreit, Stefan; Haas, Rainer; Graham, David Y; Yamaoka, Yoshio.

Affiliation

Kudo T; Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.

J Clin Microbiol ; 42(5): 2279-81, 2004 May.

Article in En | MEDLINE | ID: mdl-15131212

ABSTRACT

Polyclonal antisera to either a synthetic OipA peptide or a recombinant OipA protein detected OipA expression in Helicobacter pylori and correlated with functional oipA status determined by PCR sequence (sensitivity and specificity of >94%). Immunoblotting is a simple and accurate method for detecting expression of the important virulence factor OipA.

Subject(s)

Bacterial Proteins/genetics; Genes, Bacterial; Helicobacter pylori/genetics; Amino Acid Sequence; Antibodies, Bacterial; Bacterial Proteins/immunology; Base Sequence; DNA Primers/genetics; DNA, Bacterial/genetics; Gene Expression; Helicobacter pylori/immunology; Helicobacter pylori/pathogenicity; Humans; Immunoblotting/methods; Immunoblotting/statistics & numerical data; Molecular Sequence Data; Polymerase Chain Reaction; Recombinant Proteins/genetics; Recombinant Proteins/immunology; Sensitivity and Specificity; Virulence/genetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Helicobacter pylori / Genes, Bacterial Type of study: Diagnostic_studies Limits: Humans Language: En Journal: J Clin Microbiol Year: 2004 Type: Article Affiliation country: United States

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