Analysis of human synovial fluid phospholipase A2 on short chain phosphatidylcholine-mixed micelles: development of a spectrophotometric assay suitable for a microtiterplate reader.

Reynolds, L J; Hughes, L L; Dennis, E A

Reynolds, L J; Hughes, L L; Dennis, E A.

Affiliation

Reynolds LJ; Department of Chemistry, University of California, San Diego, La Jolla 92093-0601.

Anal Biochem ; 204(1): 190-7, 1992 Jul.

Article in En | MEDLINE | ID: mdl-1514686

Subject(s)

Phospholipases A/analysis; Spectrophotometry/methods; Synovial Fluid/enzymology; Disulfides; Dithionitrobenzoic Acid; Humans; Hydrogen-Ion Concentration; Kinetics; Micelles; Octoxynol; Phosphatidylcholines; Phospholipases A/antagonists & inhibitors; Phospholipases A2; Phospholipids/pharmacology; Polyethylene Glycols; Pyridines; Solvents; Substrate Specificity; Sulfhydryl Reagents

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Collection: 01-internacional Database: MEDLINE Main subject: Phospholipases A / Spectrophotometry / Synovial Fluid Limits: Humans Language: En Journal: Anal Biochem Year: 1992 Type: Article

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