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Anti-metastatic properties of RGD-peptidomimetic agents S137 and S247.
Shannon, Kristen E; Keene, Jeffery L; Settle, Steven L; Duffin, Tiffany D; Nickols, Maureen A; Westlin, Marisa; Schroeter, Sally; Ruminski, Peter G; Griggs, David W.
Affiliation
  • Shannon KE; Pfizer Global Research and Development, Chesterfield, Missouri 63017, USA.
Clin Exp Metastasis ; 21(2): 129-38, 2004.
Article in En | MEDLINE | ID: mdl-15168730
ABSTRACT
Integrins expressed on endothelial cells modulate cell migration and survival during angiogenesis. Integrins expressed on carcinoma cells potentiate metastasis by facilitating invasion and movement across blood vessels. We describe the activities of two synthetic low-molecular-weight peptidomimetics of the ligand amino acid sequence arg-gly-asp (RGD) in integrin-based functional assays in vitro. We also evaluate efficacy and potential mechanisms of action in models of both spontaneous and experimental metastasis. Broad-spectrum potency against the family of alpha v subunit-containing integrins was observed, with significantly less potency against alpha5beta1 and alpha(IIb)beta3. Both endothelial and tumor cell migration mediated by alpha(v)beta3 was inhibited, whereas proliferation of endothelial cells but not tumor cells was diminished. Continuous infusion of compound by minipumps or oral administration twice daily significantly reduced metastatic tumor burden in the lungs of mice despite no reduction in growth of 435/HAL primary tumors, and only a slight reduction in tumor cells detected in circulating blood. Delaying treatment in this model until after extensive dissemination of tumor cells to the lungs had occurred, and after primary tumor resection, still produced significant efficacy. Conversely, administration of the agent for only the first 18 h after tumor-cell inoculation into the tail vein also resulted in decreased metastases observed after several weeks. These data suggest these compounds or their relatives have potential to interfere with both early and late steps of metastasis involving tumor and endothelial cell functions. Furthermore, the metastatic process can be effectively inhibited independently of primary tumor growth using integrin antagonists.
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Collection: 01-internacional Database: MEDLINE Main subject: Organic Chemicals / Pyrimidines / Breast Neoplasms / Integrin alphaVbeta3 / Carcinoma, Ductal / Dipeptides / Lung Neoplasms / Neoplasm Proteins / Antineoplastic Agents Language: En Journal: Clin Exp Metastasis Journal subject: NEOPLASIAS Year: 2004 Type: Article Affiliation country: United States
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Collection: 01-internacional Database: MEDLINE Main subject: Organic Chemicals / Pyrimidines / Breast Neoplasms / Integrin alphaVbeta3 / Carcinoma, Ductal / Dipeptides / Lung Neoplasms / Neoplasm Proteins / Antineoplastic Agents Language: En Journal: Clin Exp Metastasis Journal subject: NEOPLASIAS Year: 2004 Type: Article Affiliation country: United States