Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay.
J Virol Methods
; 122(1): 29-36, 2004 Dec 01.
Article
in En
| MEDLINE
| ID: mdl-15488617
ABSTRACT
The SARS-associated human coronavirus (SARS-HCoV) is a newly described, emerging virus conclusively established as the etiologic agent of the severe acute respiratory syndrome (SARS). This study presents a single-tube RT-PCR assay that can detect with high analytical sensitivity the SARS-HCoV, as well as several other coronaviruses including other known human respiratory coronaviruses (HCoV-OC43 and HCoV-229E). Species identification is provided by sequencing the amplicon, although a rapid screening test by restriction enzyme analysis has proved to be very useful for the analysis of samples obtained during the SARS outbreak in Toronto, Canada.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Coronavirus Infections
/
Coronavirus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Severe Acute Respiratory Syndrome
/
Severe acute respiratory syndrome-related coronavirus
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
J Virol Methods
Year:
2004
Type:
Article