Analysis of yeast lipids.

Schneiter, Roger; Daum, Gunther

Schneiter, Roger; Daum, Gunther.

Affiliation

Schneiter R; Division of Biochemistry, University of Fribourg, Switzerland.

Methods Mol Biol ; 313: 75-84, 2006.

Article in En | MEDLINE | ID: mdl-16118426

ABSTRACT

ABSTRACT

The precise quantitative determination of the different lipid classes in mutant cells is key to understand the possible role of the respective gene product in lipid homeostasis. In this chapter, we describe methods based on thin-layer chromatography that are employed routinely to determine the level and relative composition of the major lipid classes from yeast.

Subject(s)

Chromatography, Thin Layer/methods; Lipids/analysis; Saccharomyces cerevisiae/chemistry; Glycerophospholipids/analysis; Glycerophospholipids/classification; Lipids/classification; Mycology/methods

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Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Chromatography, Thin Layer / Lipids Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2006 Type: Article Affiliation country: Switzerland

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