Gain-of-function screen for genes that affect Drosophila muscle pattern formation.
PLoS Genet
; 1(4): e55, 2005 Oct.
Article
in En
| MEDLINE
| ID: mdl-16254604
ABSTRACT
This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Techniques
/
Gene Expression Regulation, Developmental
/
Drosophila Proteins
/
Drosophila melanogaster
/
Muscles
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
PLoS Genet
Journal subject:
GENETICA
Year:
2005
Type:
Article
Affiliation country:
Germany