Identification and characterization of human cardiolipin synthase.
FEBS Lett
; 580(13): 3059-64, 2006 May 29.
Article
in En
| MEDLINE
| ID: mdl-16678169
ABSTRACT
The mitochondrial phospholipid cardiolipin is synthesized from cytidinediphosphate-diacylglycerol and phosphatidylglycerol, a process catalyzed by the enzyme cardiolipin synthase. In this study, we identified a human candidate gene/cDNA for cardiolipin synthase, C20orf155. Expression of this candidate cDNA in the (cardiolipin synthase-deficient) crd1Delta yeast confirmed that it indeed encodes human cardiolipin synthase. Purified mitochondria of the crd1Delta expressing human cardiolipin synthase were used to characterize the enzyme. It has an alkaline pH optimum, requires divalent cations for activity and appears to have a different substrate preference for cytidinediphosphate-diacylglycerol species when compared to phosphatidylglycerol species. The possible implications for CL synthesis and remodeling are discussed.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Transferases (Other Substituted Phosphate Groups)
/
Membrane Proteins
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
FEBS Lett
Year:
2006
Type:
Article
Affiliation country:
Netherlands