High-throughput screening methodology for the directed evolution of glycosyltransferases.

Aharoni, Amir; Thieme, Karena; Chiu, Cecilia P C; Buchini, Sabrina; Lairson, Luke L; Chen, Hongming; Strynadka, Natalie C J; Wakarchuk, Warren W; Withers, Stephen G

Aharoni, Amir; Thieme, Karena; Chiu, Cecilia P C; Buchini, Sabrina; Lairson, Luke L; Chen, Hongming; Strynadka, Natalie C J; Wakarchuk, Warren W; Withers, Stephen G.

Affiliation

Aharoni A; Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver, British Columbia V6T 1Z1, Canada.

Nat Methods ; 3(8): 609-14, 2006 Aug.

Article in En | MEDLINE | ID: mdl-16862135

Subject(s)

Escherichia coli/physiology; Evolution, Molecular; Flow Cytometry/methods; Glycosyltransferases/chemistry; Glycosyltransferases/genetics; Protein Array Analysis/methods; Sequence Analysis, Protein/methods; Amino Acid Sequence; Amino Acid Substitution; Biological Assay/methods; Biotechnology/instrumentation; Biotechnology/methods; Enzyme Activation; Flow Cytometry/instrumentation; Glycosyltransferases/analysis; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Protein Array Analysis/instrumentation

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Collection: 01-internacional Database: MEDLINE Main subject: Glycosyltransferases / Evolution, Molecular / Sequence Analysis, Protein / Protein Array Analysis / Escherichia coli / Flow Cytometry Type of study: Diagnostic_studies / Screening_studies Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2006 Type: Article Affiliation country: Canada

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