AtSNX1 defines an endosome for auxin-carrier trafficking in Arabidopsis.
Nature
; 443(7107): 106-9, 2006 Sep 07.
Article
in En
| MEDLINE
| ID: mdl-16936718
ABSTRACT
Polarized cellular distribution of the phytohormone auxin and its carriers is essential for normal plant growth and development. Polar auxin transport is maintained by a network of auxin influx (AUX) and efflux (PIN) carriers. Both auxin transport and PIN protein cycling between the plasma membrane and endosomes require the activity of the endosomal GNOM; however, intracellular routes taken by these carriers remain largely unknown. Here we show that Arabidopsis thaliana SORTING NEXIN 1 (AtSNX1) is involved in the auxin pathway and that PIN2, but not PIN1 or AUX1, is transported through AtSNX1-containing endosomes. We demonstrate that the snx1-null mutant exhibits multiple auxin-related defects and that loss of function of AtSNX1 severely enhances the phenotype of a weak gnom mutant. In root cells, we further show that AtSNX1 localizes to an endosomal compartment distinct from GNOM-containing endosomes, and that PIN2 accumulates in this compartment after treatment with the phosphatidylinositol-3-OH kinase inhibitor wortmannin or after a gravity stimulus. Our data reveal the existence of a novel endosomal compartment involved in PIN2 endocytic sorting and plant development.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endosomes
/
Carrier Proteins
/
Arabidopsis
/
Vesicular Transport Proteins
/
Indoleacetic Acids
Language:
En
Journal:
Nature
Year:
2006
Type:
Article
Affiliation country:
France