The clonal composition of human CD4+CD25+Foxp3+ cells determined by a comprehensive DNA-based multiplex PCR for TCRB gene rearrangements.

Scheinberg, Phillip; Melenhorst, Jan J; Hill, Brenna J; Keyvanfar, Keyvan; Barrett, A John; Price, David A; Douek, Daniel C

Scheinberg, Phillip; Melenhorst, Jan J; Hill, Brenna J; Keyvanfar, Keyvan; Barrett, A John; Price, David A; Douek, Daniel C.

Affiliation

Scheinberg P; Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.

J Immunol Methods ; 321(1-2): 107-20, 2007 Apr 10.

Article in En | MEDLINE | ID: mdl-17316678

Subject(s)

DNA Primers; Forkhead Transcription Factors/analysis; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor/genetics; Interleukin-2 Receptor alpha Subunit/analysis; Leukocytes, Mononuclear/immunology; Polymerase Chain Reaction/methods; Receptors, Antigen, T-Cell, alpha-beta/analysis; Adult; Amino Acid Sequence; CD4-Positive T-Lymphocytes/immunology; CD8-Positive T-Lymphocytes/immunology; Cells, Cultured; Clone Cells/immunology; Fetal Blood/cytology; Fetal Blood/immunology; Flow Cytometry; Fluorescent Antibody Technique, Indirect; Humans; Immunophenotyping/methods; Interleukin-2 Receptor alpha Subunit/genetics; Leukocytes, Mononuclear/virology; Molecular Sequence Data; Phosphoproteins/immunology; RNA Stability; Receptors, Antigen, T-Cell, alpha-beta/genetics; Reproducibility of Results; T-Lymphocytes, Regulatory/immunology; Viral Matrix Proteins/immunology

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Leukocytes, Mononuclear / Polymerase Chain Reaction / Receptors, Antigen, T-Cell, alpha-beta / Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / DNA Primers / Forkhead Transcription Factors / Interleukin-2 Receptor alpha Subunit Limits: Adult / Humans Language: En Journal: J Immunol Methods Year: 2007 Type: Article Affiliation country: United States

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