Generation of cells expressing improved doxycycline-regulated reverse transcriptional transactivator rtTA2S-M2.

Welman, Arkadiusz; Barraclough, Jane; Dive, Caroline

Welman, Arkadiusz; Barraclough, Jane; Dive, Caroline.

Affiliation

Welman A; Cancer Research UK, Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, United Kingdom. awelman@picr.man.ac.uk

Nat Protoc ; 1(2): 803-11, 2006.

Article in En | MEDLINE | ID: mdl-17406311

Subject(s)

Doxycycline/pharmacology; Gene Expression Regulation/drug effects; Genetic Engineering/methods; Trans-Activators/metabolism; Cell Line; Electroporation; Flow Cytometry; Humans; Plasmids; Trans-Activators/genetics; Transfection

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Collection: 01-internacional Database: MEDLINE Main subject: Genetic Engineering / Trans-Activators / Gene Expression Regulation / Doxycycline Limits: Humans Language: En Journal: Nat Protoc Year: 2006 Type: Article Affiliation country: United kingdom

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