Stable and unstable chromosome aberrations measured after occupational exposure to ionizing radiation and ultrasound.
Croat Med J
; 48(3): 371-7, 2007 Jun.
Article
in En
| MEDLINE
| ID: mdl-17589981
ABSTRACT
AIM:
To evaluate chromosome aberration and fluorescent in situ hybridization (FISH) assays as a method to estimate of health risk, we monitored 9 male subjects occupationally exposed to low doses of both ionizing radiation and ultrasound during a period of over 3 years.METHODS:
Sampling was performed at 6-month intervals during a three-year period. First we used conventional chromosomal aberrations analysis. When the aberration frequency for a particular subject reached the background, we measured translocations in the final sample, using fluorescence in situ hybridization. Chromosome painting probes for chromosomes 1, 2, and 4 were used simultaneously.RESULTS:
Dicentric and ring chromosomes were eliminated within a year. Translocations persisted and deviated from control values in all examinees. Translocations were detected long after unstable aberrations decreased to the background level.CONCLUSION:
Fluorescence in situ hybridization-based translocation detection was a reliable method for monitoring chronic occupational clastogen exposure. Chromosome aberration assay correlated with translocation frequency. Stable chromosomal aberrations reflected cumulative genome damage during job exposure.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ultrasonics
/
Iridium Radioisotopes
/
Occupational Exposure
/
Chromosome Aberrations
Limits:
Adult
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Croat Med J
Journal subject:
MEDICINA
Year:
2007
Type:
Article
Affiliation country:
Croatia