Your browser doesn't support javascript.
loading
Ghrelin inhibit cell apoptosis in pancreatic beta cell line HIT-T15 via mitogen-activated protein kinase/phosphoinositide 3-kinase pathways.
Zhang, Yuwei; Ying, Binwu; Shi, LiXin; Fan, Hong; Yang, Dongmei; Xu, Dan; Wei, Yonggang; Hu, Xiaobo; Zhang, Yonggang; Zhang, Xiaohong; Wang, Tao; Liu, Daishun; Dou, Liyang; Chen, Guo; Jiang, Fei; Wen, Fuqiang.
Affiliation
  • Zhang Y; Department of Endocrinology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou, Sichuan, PR China.
  • Ying B; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Shi L; Department of Endocrinology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou, Sichuan, PR China.
  • Fan H; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China; Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Yang D; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Xu D; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Wei Y; Department of General Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Hu X; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Zhang Y; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Zhang X; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Wang T; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Liu D; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Dou L; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Chen G; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Jiang F; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China.
  • Wen F; Division of Pulmonary Disease, State Key Laboratory of Biotherapy and Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, PR China. Electronic address: wenfuqiang@126.com.
Toxicology ; 237(1-3): 194-202, 2007 Jul 31.
Article in En | MEDLINE | ID: mdl-17602820
ABSTRACT
Ghrelin stimulates growth hormone (GH) release and induces positive energy balances. Previous studies have reported that ghrelin inhibits apoptosis in several cell types but the precise underlying protective mechanisms in pancreatic beta cells are poorly understood. Therefore, we investigated which pathway was related with its anti-apoptotic effect in pancreatic beta cells. Exposure of HIT-T15 cells to ghrelin caused a rapid activation of MAPKs and Akt. Chemical inhibitors of MAPK and PI3K blocked the anti-apoptotic of ghrelin. Ghrelin also stimulated the mitochondrial pathways of apoptosis and it showed increased Bcl-2, decreased Bax, prevention cytochrome c release and inhibition of caspase-3 activation in pancreatic beta cell line HIT-T15. Our findings suggest that ghrelin may act as a survival factor that inhibits the apoptotics pathways, and the MAPKs, AKT pathways could be key roles in the apoptosis of pancreatic beta cells.
Subject(s)
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Apoptosis / Phosphatidylinositol 3-Kinases / Mitogen-Activated Protein Kinases / Peptide Hormones / Insulin-Secreting Cells Limits: Animals Language: En Journal: Toxicology Year: 2007 Type: Article
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Apoptosis / Phosphatidylinositol 3-Kinases / Mitogen-Activated Protein Kinases / Peptide Hormones / Insulin-Secreting Cells Limits: Animals Language: En Journal: Toxicology Year: 2007 Type: Article