Encapsulation and diffraction-pattern-correction methods to reduce the effect of damage in x-ray diffraction imaging of single biological molecules.
Phys Rev Lett
; 98(19): 198302, 2007 May 11.
Article
in En
| MEDLINE
| ID: mdl-17677667
ABSTRACT
Short and intense x-ray pulses may be used for atomic-resolution diffraction imaging of single biological molecules. Radiation damage and a low signal-to-noise ratio impose stringent pulse requirements. In this Letter, we describe methods for decreasing the damage and improving the signal by encapsulating the molecule in a sacrificial layer (tamper) that reduces atomic motion and by postprocessing the pulse-averaged diffraction pattern to correct for ionization damage. Simulations show that these methods greatly improve the image quality.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biophysics
/
Computer Simulation
/
Models, Theoretical
Language:
En
Journal:
Phys Rev Lett
Year:
2007
Type:
Article
Affiliation country:
United States