Up-converting phosphor technology-based lateral flow assay for detection of Schistosoma circulating anodic antigen in serum.
J Clin Microbiol
; 46(1): 171-6, 2008 Jan.
Article
in En
| MEDLINE
| ID: mdl-17942645
ABSTRACT
Schistosoma sp. circular anodic antigen (CAA) serum concentrations reflect actual worm burden in a patient and are a valuable tool for population screening and epidemiological research. However, for the diagnosis of individual imported schistosomiasis cases, the current enzyme-linked immunosorbent assay (ELISA) lacks sensitivity and robustness. Therefore, a lateral flow (LF) assay was developed to test CAA in serum for individual diagnosis of imported active schistosome infections. Application of fluorescent submicron-sized up-converting phosphor technology (UPT) reporter particles increased analytical sensitivity compared to that of the standard ELISA method. Evaluation of the UPT-LF test with a selection of 40 characterized epidemiologic samples indicated a good correlation between signal intensity and infection intensity. Subsequently, the UPT-LF assay was applied to 166 serum samples of Dutch residents (immigrants and travelers) suspected of schistosomiasis, a case in which group routine antibody detection frequently fails straightforward diagnosis. The UPT-LF assay identified 36 CAA-positive samples, compared to 15 detected by CAA-ELISA. In conclusion, the UPT-LF assay is a low-complexity test with higher sensitivity than the CAA-ELISA, well suited for laboratory diagnosis of individual active Schistosoma infections.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Parasitology
/
Schistosoma
/
Schistosomiasis
/
Antigens, Helminth
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limits:
Animals
/
Humans
Country/Region as subject:
Europa
Language:
En
Journal:
J Clin Microbiol
Year:
2008
Type:
Article
Affiliation country:
Netherlands