In vitro- and in vivo-induced transgene expression in human embryonic stem cells and derivatives.
Stem Cells
; 26(2): 525-33, 2008 Feb.
Article
in En
| MEDLINE
| ID: mdl-18032700
ABSTRACT
The use of human embryonic stem cells (hESCs) as a research and therapeutic tool will be facilitated by conditional gene expression. Here, we report drug-induced transgene expression, both in vitro and in vivo, from a tet-on hESC line with >95% purity. Using green fluorescent protein as an indicator, we demonstrated that the tet-on system allowed a tight control of the gene expression in both undifferentiated hESCs and differentiated cells of the three germ layers. More importantly, after the cells were transplanted into animals, the gene expression remained to be regulated by an orally administered drug. These results provide a technical basis for regulation of gene expression in hESCs and derivatives in vitro and in vivo.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Embryonic Stem Cells
Type of study:
Etiology_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Stem Cells
Year:
2008
Type:
Article
Affiliation country:
United States