Effect of 1-methyladenine on double-helical DNA structures.
FEBS Lett
; 582(11): 1629-33, 2008 May 14.
Article
in En
| MEDLINE
| ID: mdl-18435925
ABSTRACT
Methylation at the N1 site of adenine leads to the formation of cytotoxic 1-methyladenine (m1A). Since the N1 site of adenine is involved in the hydrogen bonding of T.A and A.T Watson-Crick base pairs, it is expected that the pairing interactions will be disrupted upon 1-methylation. In this study, high-resolution NMR investigations were performed to determine the effect of m1A on double-helical DNA structures. Interestingly, instead of disrupting hydrogen bonding, we found that 1-methylation altered the T.A Watson-Crick base pair to T(anti).m1A(syn) Hoogsteen base pair, providing insights into the observed differences in AlkB-repair efficiency between dsDNA and ssDNA.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Adenine
/
DNA Methylation
/
Base Pairing
/
Nucleic Acid Conformation
Language:
En
Journal:
FEBS Lett
Year:
2008
Type:
Article
Affiliation country:
Hong Kong