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Micro biochemical engineering to accelerate the design of industrial-scale downstream processes for biopharmaceutical proteins.
Titchener-Hooker, N J; Dunnill, P; Hoare, M.
Affiliation
  • Titchener-Hooker NJ; Department of Biochemical Engineering, Advanced Centre for Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
Biotechnol Bioeng ; 100(3): 473-87, 2008 Jun 15.
Article in En | MEDLINE | ID: mdl-18438873
The article examines how a small set of easily implemented micro biochemical engineering procedures combined with regime analysis and bioprocess models can be used to predict industrial scale performance of biopharmaceutical protein downstream processing. This approach has been worked on in many of our studies of individual operations over the last 10 years and allows preliminary evaluation to be conducted much earlier in the development pathway because of lower costs. It then permits the later large scale trials to be more highly focused. This means that the risk of delays during bioprocess development and of product launch are reduced. Here we draw the outcomes of this research together and illustrate its use in a set of typical operations; cell rupture, centrifugation, filtration, precipitation, expanded bed adsorption, chromatography and for common sources, E. coli, two yeasts and mammalian cells (GS-NSO). The general approach to establishing this method for other operations is summarized and new developments outlined. The technique is placed against the background of the scale-down methods that preceded it and complementary ones that are being examined in parallel. The article concludes with a discussion of the advantages and limitations of the micro biochemical engineering approach versus other methods.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Products / Biotechnology / Recombinant Proteins / Chemical Engineering / Microchemistry Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Biotechnol Bioeng Year: 2008 Type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biological Products / Biotechnology / Recombinant Proteins / Chemical Engineering / Microchemistry Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Biotechnol Bioeng Year: 2008 Type: Article