Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study.
J Chromatogr B Analyt Technol Biomed Life Sci
; 875(1): 161-7, 2008 Nov 01.
Article
in En
| MEDLINE
| ID: mdl-18514599
ABSTRACT
An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively, and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mandelic Acids
Limits:
Animals
Language:
En
Journal:
J Chromatogr B Analyt Technol Biomed Life Sci
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2008
Type:
Article
Affiliation country:
Brazil