Dissemination of Cronobacter spp. (Enterobacter sakazakii) in a powdered milk protein manufacturing facility.

Mullane, N; Healy, B; Meade, J; Whyte, P; Wall, P G; Fanning, S

Mullane, N; Healy, B; Meade, J; Whyte, P; Wall, P G; Fanning, S.

Affiliation

Mullane N; Centre for Food Safety, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland.

Appl Environ Microbiol ; 74(19): 5913-7, 2008 Oct.

Article in En | MEDLINE | ID: mdl-18641152

ABSTRACT

ABSTRACT

The microbial contamination of air filters and possible links to contaminated product in a powdered milk protein-processing facility were investigated. Over a 10-month period, seven air filters, the environment, and powdered product were analyzed for the presence of Cronobacter spp. The effects of air filter installation, maintenance, and subsequent dissemination of Cronobacter were investigated. A total of 30 isolates were characterized by pulsed-field gel electrophoresis (PFGE). PFGE revealed the presence of three clonal populations distributed throughout the manufacturing site. This study highlights the need for proper installation of air filters to limit the dissemination of microorganisms into processing sites.

Subject(s)

Cronobacter sakazakii/classification; Cronobacter sakazakii/isolation & purification; Environmental Microbiology; Food Contamination; Food Microbiology; Bacterial Typing Techniques; Cluster Analysis; Cronobacter sakazakii/genetics; DNA Fingerprinting; DNA, Bacterial/genetics; Electrophoresis, Gel, Pulsed-Field; Genotype; Milk Proteins

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Food Contamination / Cronobacter sakazakii / Environmental Microbiology / Food Microbiology Language: En Journal: Appl Environ Microbiol Year: 2008 Type: Article Affiliation country: Ireland

Fulltext

XML

PubMed Links

Search on Google