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Simultaneous determination of three anticonvulsants using hydrophilic interaction LC-MS.
Oertel, Reinhard; Arenz, Norman; Pietsch, Jörg; Kirch, Wilhelm.
Affiliation
  • Oertel R; Institute of Clinical Pharmacology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Dresden, Germany. reinhard.oertel@tu-dresden.de
J Sep Sci ; 32(2): 238-43, 2009 Jan.
Article in En | MEDLINE | ID: mdl-19072899
A specific and automated method was developed to quantify the anticonvulsants gabapentin, pregabalin and vigabatrin simultaneously in human serum. Samples were prepared with a protein precipitation. The hydrophilic interaction chromatography (HILIC) with a mobile phase gradient was used to divide off ions of the matrix and for separation of the analytes. Four different HILIC-columns and two different column temperatures were tested. The Tosoh-Amid column gave the best results: single small peaks. The anticonvulsants were detected in the multiple reaction monitoring mode (MRM) with ESI-MS-MS. Using a volume of 100 microL biological sample the lowest point of the standard curve, i.e. the lower LOQs were 312 ng/mL. The described HILIC-MS-MS method is suitable for therapeutic drug monitoring and for clinical and pharmcokinetical investigations of the anticonvulsives.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, Liquid / Vigabatrin / Cyclohexanecarboxylic Acids / Spectrometry, Mass, Electrospray Ionization / Tandem Mass Spectrometry / Gamma-Aminobutyric Acid / Amines / Anticonvulsants Language: En Journal: J Sep Sci Year: 2009 Type: Article Affiliation country: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, Liquid / Vigabatrin / Cyclohexanecarboxylic Acids / Spectrometry, Mass, Electrospray Ionization / Tandem Mass Spectrometry / Gamma-Aminobutyric Acid / Amines / Anticonvulsants Language: En Journal: J Sep Sci Year: 2009 Type: Article Affiliation country: Germany