Your browser doesn't support javascript.
loading
The twin spot generator for differential Drosophila lineage analysis.
Griffin, Ruth; Sustar, Anne; Bonvin, Marianne; Binari, Richard; del Valle Rodriguez, Alberto; Hohl, Amber M; Bateman, Jack R; Villalta, Christians; Heffern, Elleard; Grunwald, Didier; Bakal, Chris; Desplan, Claude; Schubiger, Gerold; Wu, C-ting; Perrimon, Norbert.
Affiliation
  • Griffin R; Commissariat à l'Energie Atomique, Institut de Recherches en Technologies et Sciences pour le Vivant, Centre National de la Recherche Scientifique, Grenoble, France. rgriffin@receptor.med.harvard.edu
Nat Methods ; 6(8): 600-2, 2009 Aug.
Article in En | MEDLINE | ID: mdl-19633664
ABSTRACT
In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.
Subject(s)
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombination, Genetic / Cell Lineage / Genomics / Drosophila melanogaster / Mitosis Limits: Animals Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2009 Type: Article Affiliation country: France
Fulltext
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombination, Genetic / Cell Lineage / Genomics / Drosophila melanogaster / Mitosis Limits: Animals Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2009 Type: Article Affiliation country: France