Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens.

Yang, Genyan Patrick; Erdman, Dean D; Tondella, Maria L; Fields, Barry S

Yang, Genyan Patrick; Erdman, Dean D; Tondella, Maria L; Fields, Barry S.

Affiliation

Yang GP; Centers for Disease Control and Prevention, Division of Bacterial Diseases, Respiratory Diseases Branch, Atlanta, GA 30333, USA. gyang@cdc.gov

J Virol Methods ; 162(1-2): 288-90, 2009 Dec.

Article in En | MEDLINE | ID: mdl-19699237

Subject(s)

DNA Probes; Fluorescent Dyes; Reagent Kits, Diagnostic; Respiratory System; Reverse Transcriptase Polymerase Chain Reaction/methods; Rhodamines; Bacteria/classification; Bacteria/genetics; Bacteria/isolation & purification; Bacterial Infections/virology; Fluorescent Dyes/classification; Fluorescent Dyes/metabolism; Humans; Respiratory System/microbiology; Respiratory System/virology; Rhodamines/metabolism; Taq Polymerase; Virus Diseases/virology; Viruses/classification; Viruses/genetics; Viruses/isolation & purification

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Reagent Kits, Diagnostic / Respiratory System / Rhodamines / DNA Probes / Reverse Transcriptase Polymerase Chain Reaction / Fluorescent Dyes Type of study: Evaluation_studies Limits: Humans Language: En Journal: J Virol Methods Year: 2009 Type: Article Affiliation country: United States

Fulltext

XML

PubMed Links

Search on Google