Characterization of binding affinities in a chromatographic system by suspended state HR/MAS NMR spectroscopy.
Magn Reson Chem
; 48(2): 111-6, 2010 Feb.
Article
in En
| MEDLINE
| ID: mdl-19950216
ABSTRACT
In the current work a racemate of (R)- and (S)-benzylmandelate was separated with a stereoselective polysaccharide-based chiral stationary phase by HPLC. To elucidate the occurring chiral molecular recognition processes in the heterogeneous system used, NMR spectroscopy was chosen under high resolution/magic angle spinning (HR/MAS) NMR conditions in the suspended state. Therefore, and as a proof of concept, a combination of several NMR methods such as spin-lattice relaxation time (T(1)) measurements (T(1)), the saturation transfer difference, and the 2D experiment of the transferred nuclear overhauser enhancement spectroscopy technique were applied. With HR/MAS NMR it is feasible to combine NMR and chromatography to achieve further insights into the separation process.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mandelic Acids
Language:
En
Journal:
Magn Reson Chem
Journal subject:
QUIMICA
Year:
2010
Type:
Article
Affiliation country:
Germany