Structure-based engineering of a monoclonal antibody for improved solubility.

Wu, Sheng-Jiun; Luo, Jinquan; O'Neil, Karyn T; Kang, James; Lacy, Eilyn R; Canziani, Gabriela; Baker, Audrey; Huang, Maggie; Tang, Qing Mike; Raju, T Shantha; Jacobs, Steven A; Teplyakov, Alexey; Gilliland, Gary L; Feng, Yiqing

Wu, Sheng-Jiun; Luo, Jinquan; O'Neil, Karyn T; Kang, James; Lacy, Eilyn R; Canziani, Gabriela; Baker, Audrey; Huang, Maggie; Tang, Qing Mike; Raju, T Shantha; Jacobs, Steven A; Teplyakov, Alexey; Gilliland, Gary L; Feng, Yiqing.

Affiliation

Wu SJ; Biologics Research, Centocor R&D, 145 King of Prussia Radnor, PA 19087-4557, USA. swu4@its.jnj.com

Protein Eng Des Sel ; 23(8): 643-51, 2010 Aug.

Article in En | MEDLINE | ID: mdl-20543007

Subject(s)

Antibodies, Monoclonal/chemistry; Protein Conformation; Protein Engineering/methods; Protein Stability; Amino Acid Sequence; Antibodies, Monoclonal/genetics; Antibodies, Monoclonal/metabolism; Binding Sites; Calorimetry, Differential Scanning; Electrophoresis, Polyacrylamide Gel; Humans; Hydrophobic and Hydrophilic Interactions; Interleukin-13/antagonists & inhibitors; Interleukin-13/metabolism; Isoelectric Focusing; Isoelectric Point; Models, Molecular; Molecular Sequence Data; Peptide Mapping; Protein Multimerization; Solubility; Temperature

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Conformation / Protein Engineering / Protein Stability / Antibodies, Monoclonal Limits: Humans Language: En Journal: Protein Eng Des Sel Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2010 Type: Article Affiliation country: United States

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