Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents.

Hancock, W S; Bishop, C A; Prestidge, R L; Harding, D R; Hearn, M T

Hancock, W S; Bishop, C A; Prestidge, R L; Harding, D R; Hearn, M T.

Science ; 200(4346): 1168-70, 1978 Jun 09.

Article in En | MEDLINE | ID: mdl-206966

ABSTRACT

ABSTRACT

Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).

Subject(s)

Chromatography, High Pressure Liquid/methods; Oligopeptides/isolation & purification; Peptide Fragments/isolation & purification; Proteins/isolation & purification; Adrenocorticotropic Hormone/isolation & purification; Carrier Proteins/isolation & purification; Glucagon/isolation & purification; Insulin/isolation & purification; Phosphates; Solvents

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Collection: 01-internacional Database: MEDLINE Main subject: Oligopeptides / Peptide Fragments / Proteins / Chromatography, High Pressure Liquid Language: En Journal: Science Year: 1978 Type: Article

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