Acetylcholinesterase inhibitory activity of uleine from Himatanthus lancifolius.
Z Naturforsch C J Biosci
; 65(7-8): 440-4, 2010.
Article
in En
| MEDLINE
| ID: mdl-20737911
ABSTRACT
Application of acetylcholinesterase (AChE) inhibitors is the primary treatment for Alzheimer's disease. Alkaloids, such as physostigmine, galanthamine, and huperzine A, play an important role as AChE inhibitors. The aim of this work was to evaluate Himatanthus lancifolius (Muell. Arg.) Woodson, a Brazilian species of Apocynaceae, and its main indole alkaloid uleine, in order to identify new AChE inhibitors. The plant fluid extract, fractions, and uleine were tested for AChE inhibitory activity using Ellman's colorimetric method for thin-layer chromatography (TLC), 96-well microplates, and also Marston's TLC colorimetric method. Both TLC assays showed similar results. At 5 mg/mL, the fluid extract inhibited the AChE enzyme by (50.71 +/- 8.2)%. The ethyl acetate fraction exhibited the highest level of AChE inhibition, followed by the dichloromethane fraction. The isolated alkaloid uleine displayed an IC50 value of 0.45 microM.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bridged-Ring Compounds
/
Cholinesterase Inhibitors
/
Apocynaceae
/
Alkaloids
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Z Naturforsch C J Biosci
Year:
2010
Type:
Article
Affiliation country:
Brazil