Integration of bovine herpesvirus 4 genome into cultured persistently infected host cell genome.
Virol J
; 7: 246, 2010 Sep 21.
Article
in En
| MEDLINE
| ID: mdl-20854697
ABSTRACT
Persistent infection of macrophages with bovine herpesvirus 4 (BoHV-4) has been proposed to play a secondary causal role, along with bacterial infection, in bovine post-partum metritis. Mechanisms of maintenance of BoHV-4 persistent infection are not understood. We previously generated in vitro models of BoHV-4 persistent infection in human rhadomyosarcoma and bovine macrophage cell lines by drug selection of cells infected with BoHV-4 carrying a drug-resistance marker, and demonstrated circular episomal BoHV-4 genomes. In the present study, we used fluorescent in situ hybridization (FISH) to demonstrate BoHV-4 genomes also integrated into the genomes of these persistently infected cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Virus Integration
/
Herpesvirus 4, Bovine
Limits:
Animals
Language:
En
Journal:
Virol J
Journal subject:
VIROLOGIA
Year:
2010
Type:
Article
Affiliation country:
Italy