Exogenous procalcitonin evokes a pro-inflammatory cytokine response.
Inflamm Res
; 60(2): 203-7, 2011 Feb.
Article
in En
| MEDLINE
| ID: mdl-20953970
ABSTRACT
OBJECTIVE AND DESIGN:
Procalcitonin (ProCT) is increased in serum of septic patients and those with systemic inflammation. Endogenous levels of ProCT might influence the response of polymorphonuclear leukocytes (PMNs), independently of endotoxin, in clinical disease.SUBJECTS:
Healthy human volunteers. TREATMENT Recombinant human ProCT (rhProCT).METHODS:
Whole blood and PMNs were exposed in vitro to exogenous rhProCT. Interleukin (IL)-6, IL-8, IL-10, IL-13, tumor necrosis factor-alpha (TNFα), IL-1ß, and macrophage inflammatory protein (MIP)-1ß (pg/ml) were measured by multiplex suspension bead-array immunoassay, and migration and phagocytosis were measured in PMNs.RESULTS:
In a whole-blood model, a dose-dependent increase in IL-6, TNFα, and IL-1ß of the cell-free supernatant was noted. Pre-incubation with ProCT, at doses consistent with clinical sepsis, resulted in a decrease in PMN migration without alteration in phagocytosis of Staphylococcus aureus or indirect measurements of bacterial killing.CONCLUSION:
Clinically relevant levels of ProCT influence immunologic responses that may contribute to systemic inflammatory response and septic shock.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Precursors
/
Calcitonin
/
Cytokines
/
Inflammation
/
Neutrophils
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Inflamm Res
Journal subject:
ALERGIA E IMUNOLOGIA
/
PATOLOGIA
Year:
2011
Type:
Article
Affiliation country:
United States