Lactose does not interfere with the analysis of sialic acids as their 1,2-diamino-4,5-methylenedioxybenzene derivatives.
Anal Bioanal Chem
; 399(5): 1917-22, 2011 Feb.
Article
in En
| MEDLINE
| ID: mdl-21161511
ABSTRACT
In 2007, Martin et al. developed a method for the analysis of sialic acids by HPLC following 1,2-diamino-4,5-methylenedioxybenzene (DMB) derivatisation (Martín et al., Anal Bioanal Chem 3872943-2949, 2007). Within the article, the authors noted that lactose interfered with the analysis, giving erroneously high results when lactose-containing products were analysed. Such an observation is important when analysing milk-based products, yet was contradictory to the observations of Nakamura et al. (Chem Pharm Bull 35(2)687-692, 1987) who demonstrated that DMB was specific for α-keto acids and did not react with simple sugars such as glucose or lactose. In order to clarify the situation, this phenomenon was investigated and it was confirmed that lactose does not interfere with the analysis. However, it was found that most commercial preparations of lactose do contain small amounts of sialic acids, either as the free monosaccharide or bound to lactose in the form of 3'- and 6'-sialyllactose.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenylenediamines
/
Sialic Acids
/
Lactose
Language:
En
Journal:
Anal Bioanal Chem
Year:
2011
Type:
Article
Affiliation country:
Switzerland