Characterization of complementary chromatic adaptation in Gloeotrichia UTEX 583 and identification of a transposon-like insertion in the cpeBA operon.
Plant Cell Physiol
; 52(3): 553-62, 2011 Mar.
Article
in En
| MEDLINE
| ID: mdl-21288891
ABSTRACT
Many cyanobacteria are able to alter the pigment composition of the phycobilisome in a process called complementary chromatic adaptation (CCA). The regulatory mechanisms of CCA have been identified in Fremyella diplosiphon, which regulates both phycoerythrin and phycocyanin levels, and Nostoc punctiforme, which regulates only phycoerythrin production. Recent studies show that these species use different regulatory proteins for CCA. We chose to study the CCA response of Gloeotrichia UTEX 583 in an effort to expand our knowledge about CCA and its regulation. We found that Gloeotrichia 583 has a CCA pigment response more similar to that of N. punctiforme rather than F. diplosiphon and exhibits none of the CCA-regulated morphological responses seen in F. diplosiphon. Preliminary experiments suggest that Gloeotrichia 583 contains a homolog to the CCA photoreceptor from N. punctiforme but not the CCA photoreceptor from F. diplosiphon. Additionally, two spontaneous mutants lacking phycoerythrin production were identified. Analysis has shown that these mutants contain a transposon-like insertion in the cpeA gene, which encodes the α subunit of phycoerythrin. These results suggest that CCA in Gloeotrichia UTEX 583 is more similar to that of N. punctiforme than it is to F. diplosiphon, a closely related species.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Operon
/
Adaptation, Physiological
/
DNA Transposable Elements
/
Mutagenesis, Insertional
/
Cyanobacteria
/
Light
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
Plant Cell Physiol
Journal subject:
BOTANICA
Year:
2011
Type:
Article
Affiliation country:
United States