Expression and purification of full-length anti-apoptotic Bcl-2 using cell-free protein synthesis.

Pedersen, Anders; Wallgren, Marcus; Karlsson, B Göran; Gröbner, Gerhard

Pedersen, Anders; Wallgren, Marcus; Karlsson, B Göran; Gröbner, Gerhard.

Affiliation

Pedersen A; Swedish NMR Centre, University of Gothenburg, Gothenburg, Sweden.

Protein Expr Purif ; 77(2): 220-3, 2011 Jun.

Article in En | MEDLINE | ID: mdl-21315822

Subject(s)

Protein Isoforms/biosynthesis; Proto-Oncogene Proteins c-bcl-2/biosynthesis; Recombinant Fusion Proteins/biosynthesis; Subcellular Fractions/metabolism; Apoptosis; Cell Fractionation; Cell-Free System; Cetomacrogol/chemistry; Chromatography, Affinity; Circular Dichroism; Cloning, Molecular; Escherichia coli; Humans; Hydrophobic and Hydrophilic Interactions; Leukemia, Lymphocytic, Chronic, B-Cell/genetics; Leukemia, Lymphocytic, Chronic, B-Cell/metabolism; Lymphoma, B-Cell/genetics; Lymphoma, B-Cell/metabolism; Protein Biosynthesis; Protein Folding; Protein Isoforms/genetics; Protein Isoforms/isolation & purification; Protein Structure, Secondary; Proto-Oncogene Proteins c-bcl-2/genetics; Proto-Oncogene Proteins c-bcl-2/isolation & purification; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/isolation & purification; Solubility; Subcellular Fractions/chemistry

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Subcellular Fractions / Recombinant Fusion Proteins / Proto-Oncogene Proteins c-bcl-2 / Protein Isoforms Type of study: Guideline Limits: Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2011 Type: Article Affiliation country: Sweden

Fulltext

XML

PubMed Links

Search on Google