Poliovirus serotype-specific VP1 sequencing primers.
J Virol Methods
; 174(1-2): 128-30, 2011 Jun.
Article
in En
| MEDLINE
| ID: mdl-21440569
ABSTRACT
The Global Polio Laboratory Network routinely uses poliovirus-specific PCR primers and probes to determine the serotype and genotype of poliovirus isolates obtained as part of global poliovirus surveillance. To provide detailed molecular epidemiologic information, poliovirus isolates are further characterized by sequencing the ~900-nucleotide region encoding the major capsid protein, VP1. It is difficult to obtain quality sequence information when clinical or environmental samples contain poliovirus mixtures. As an alternative to conventional methods for resolving poliovirus mixtures, sets of serotype-specific primers were developed for amplifying and sequencing the VP1 regions of individual components of mixed populations of vaccine-vaccine, vaccine-wild, and wild-wild polioviruses.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
DNA Primers
/
Poliovirus
/
Capsid Proteins
Type of study:
Diagnostic_studies
/
Evaluation_studies
Limits:
Humans
Language:
En
Journal:
J Virol Methods
Year:
2011
Type:
Article
Affiliation country:
United States