Signaling molecules for early detection of adverse interactions during mechanical ventilation in animal models.
In Vivo
; 25(2): 209-17, 2011.
Article
in En
| MEDLINE
| ID: mdl-21471537
ABSTRACT
AIM:
The early identification of adverse interactions during mechanical ventilation, investigated by multiplexed immunoanalysis. MATERIALS ANDMETHODS:
Twenty piglets (average age 7 weeks, weight 23 kg) were intubated and divided into groups A, spontaneously breathing; B, protectively ventilated; C, ventilated with injurious strategy; D, ventilated with lung disability. At the 1st hour (time-1) and 12th hour (time-2) of the study, brain natriuretic peptide (BNP), intercellular cell adhesion molecules (ICAM-1), vascular cell adhesion molecules (VCAM-1), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (Il-6) were analyzed in the blood.RESULTS:
The injurious ventilated group C exhibited an increase in both cell adhesion molecules (p<0.01), TNF-alpha and BNP (p<0.05) at time-1, and at time-2 further increases (p<0.05). In group D, an increase in ICAM-1 and BNP (p<0.05) at time-1, and increases in Il-6 and ICAM-1 (p<0.05) at time-2, with notable decreases in urine output were observed. Overall, the lung damage correlated with TNF-alpha (r=0.904), Il-6 (r=0.740), and ICAM-1 (r=0.756) levels.CONCLUSION:
All five monitored molecules quickly and reliably signaled adverse interactions.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Respiration, Artificial
/
Biomarkers
/
Lung Injury
/
Multiple Organ Failure
Type of study:
Diagnostic_studies
/
Etiology_studies
/
Prognostic_studies
/
Screening_studies
Limits:
Animals
Language:
En
Journal:
In Vivo
Journal subject:
NEOPLASIAS
Year:
2011
Type:
Article
Affiliation country:
Czech Republic