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Prokaryotic expression and identification of 3-1E gene of merozoite surface antigen of Eimeria acervulina.
Zhao, Yuelan; Wang, Chengmin; Lu, Yanmin; Amer, Said; Xu, Ping; Wang, Jianyong; Lu, Junxia; Bao, Yongzhan; Deng, Bolin; He, Hongxuan; Qin, Jianhua.
Affiliation
  • Zhao Y; College of Chinese Traditional Veterinary Science, Hebei Agriculture University, Dingzhou, China.
Parasitol Res ; 109(5): 1361-5, 2011 Nov.
Article in En | MEDLINE | ID: mdl-21499749
Eimeria acervulina was isolated from chicken at Hebei province, China. The gene of merozoite surface antigen 3-1E was amplified and cloned into pET28a(+) vector and then transformed into Escherichia coli BL21 strain. Results showed that 3-1E fusion protein band of about 22 kDa was identified by SDS-PAGE. Western blot analysis indicated that the recombinant protein specifically reacted with E. acervulina polyclonal antibody.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protozoan Proteins / Eimeria / Antigens, Protozoan / Antigens, Surface Type of study: Diagnostic_studies Limits: Animals Country/Region as subject: Asia Language: En Journal: Parasitol Res Journal subject: PARASITOLOGIA Year: 2011 Type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protozoan Proteins / Eimeria / Antigens, Protozoan / Antigens, Surface Type of study: Diagnostic_studies Limits: Animals Country/Region as subject: Asia Language: En Journal: Parasitol Res Journal subject: PARASITOLOGIA Year: 2011 Type: Article Affiliation country: China