Analysis of multilocus sequence typing schemes for 35 different bacteria revealed that gene loci of 10 bacteria could be replaced to improve cost-effectiveness.
Diagn Microbiol Infect Dis
; 70(3): 316-23, 2011 Jul.
Article
in En
| MEDLINE
| ID: mdl-21558049
ABSTRACT
Although multilocus sequence typing (MLST) has been widely used for bacterial typing, the contribution of the gene loci to the discriminatory power of each MLST scheme is unknown. We analyzed the discriminatory powers of 36 MLST schemes using all combinations of the 7 loci and contributions of each locus to the schemes. In 10 schemes, sequencing 6 loci can achieve the discriminatory powers of 7 loci. For the other 26 schemes, the median marginal increase in discriminatory power when 7 instead of 6 loci were used is 0.0004. Sequencing the 7 loci of 50 strains each of Pseudomonas aeruginosa and Acinetobacter baumannii revealed that the discriminatory power for P. aeruginosa was 0.9861 when either 6 (without trp) or 7 loci were used and that for A. baumannii was 0.9363 when 5, 6, or 7 loci were used. Genes that have no additional or minimal contribution to the overall discriminatory powers should be replaced.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pseudomonas aeruginosa
/
Acinetobacter baumannii
/
Multilocus Sequence Typing
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Health_economic_evaluation
Limits:
Humans
Language:
En
Journal:
Diagn Microbiol Infect Dis
Year:
2011
Type:
Article
Affiliation country:
Hong Kong